Ability difference of Beluntas Leaf (Pluchea indica L) ethanol extract and avocado leaf (Persea americana Mill) ethanol extract in Inhibiting caries-causing Streptococcus mutans Bacteria Growth

Maria Martina Nahak; Regina Tedjasulaksana; Ni Nengah Sumerti

doi:10.15562/bmj.v6i3.742

Ability difference of Beluntas Leaf (Pluchea indica L) ethanol extract and avocado leaf (Persea americana Mill) ethanol extract in Inhibiting caries-causing Streptococcus mutans Bacteria Growth

Maria Martina Nahak ,

Regina Tedjasulaksana ,

Ni Nengah Sumerti ,

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DOI: https://doi.org/10.15562/bmj.v6i3.742 |
Published: 2017-09-06

Abstract

Beluntas Leaf (Pluchea indica L) has been shown to have antibacterial activity against Streptococcus mutans. The leaf of avocado (Persea americana Mill) that have same active substance with beluntas leaf expected to have antibacterial activity against Streptococcus mutans so that the purpose of this research is to compare the ability of ethanol extract from avocado leaf and beluntas leaf in inhibiting the growth of Streptococcus mutans. This research is experimental type, using completely randomized with posttest only control group design. Samples were pure isolates of Streptococcus mutans ATCC 35668. The sample of 90 divided into four groups: beluntas leaf ethanol extract 25%, beluntas leaf ethanol extract 50%, avocado leaf ethanol extract 25%, avocado leaf ethanol extract 50% and two control groups. The data were analyzed using One-Way ANOVA test followed by Least Significant Difference (LSD) with a 95% confidence level. The results showed a significant inhibition differences between the control group and the treatment group with a value of p = 0.000 (p <0.05). The ability of 25% ethanol extract of beluntas leaf (Pluchea indica L) is equivalent to 50% ethanol extract of beluntas leaf (Pluchea indica L) and 50% ethanol extract of leaf of avocado (Persea americana Mill) in inhibiting the growth of Streptococcus mutans. It is recommended that further research conducted to test the mechanism of action of the active substances contained in beluntas and avocado leaf as an antibacterial and test MIC (Minimum Inhibitory Concentration) with an advanced test in determining the MBC (Minimal Bactericidal Concentration).

References

Vinogradov, A.M., Winston, M., Rupp, C.J., Stoodley, P. 2004. Rheology of Biofilms Formed from the Dental Plaque Pathogen Streptococcus mutans. Biofilm 1: 49-56
ArgimÃµn, S., Caufiled, P.W. 2011. Distribution of Putative Virulence genes in Streptococcus mutans Strain does not Correlate with Caries Experience. Journal of Clinical Microbiology. 49(3): 984-92
Quock, R.L. Dental Caries : A Current Understanding and Implications. Journal of Nature Science, Vol.1, e27, 2015 : 1-3
Shahani, M.N., Reddy, V.V.S. 2011. Comparison of Antimicrobial Substantivity of Root Canal Irrigants in Instrumented Root Canals up to 72 Hours: An Invitro Study. Journal of Indian Soc. Pedod. Prev. Dent. 29: 28-33
Menendez, A., Li, F., Michalek, S., Kirk, K., Makhija, S.K., Childers, N.K. 2005. Comparative Analysis of the Antibacterial Effects of Combined Mouthrinses on Streptococcus mutans. Journal of Oral Microbiology and Immunology; 20(1): 31
Lyle D. The Role of Pharmacotherapeutics in the Reduction of Plaque and Gingivitis. J Prac Hyg. 2000;Suppl9(6): 46-50
Andarwulan, N., Batari, R., Agustini, D., Bolling, B., Wijaya, H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chemistry. 2010;121: 1231-1235.
Ahemd, S.A., Kamel, E.M. Phenolic constituents and biological activity of the genus Pluchea. Der Pharma Chemica. 2013;5(5): 109-114.
Hussain, A., Wahab, S., Zarin, I., Sarfaraj Hussain, M.D. 2010. Antibacterial Activity of the Leaves of Cocconia indica (W. and A) Wof India. Advances in Biological Research. 4(5): 241-248
Mohamed, S.S.H., Hansi, P.D., Thirumurugan, K. 2010. Antimicrobial Activity and Phytochemical Analysis of Selected Indian Folk Medicinal Plants. International Journal of Pharma Sciences and Research (IJPSR). 1(10): 430-434
Devi, A.P. 2011. Korelasi Kadar Fenolat Daun Dendrophthoe petandra, Dendrophthoe falcata, dan Scurrula philippensis Terhadap Aktivitas Antioksidan dengan Metode DPPH. Skripsi. Univerversitas Muhammadiyah. Surakarta. p. 1-15
Machado, T.B., Pinto, A.V., Pinto, M.C.F.R., Leal, I.C.R., Silva, M.G., Amaral, A.C.F., Kuster, R.M., Netto-dos Santoz, K.R. 2003. In vitro Activity of Brazilian Medicinal Plants, Naturally Occuring Naphtoquinones and Their Analogues Against Methicilline Resistant Staphyloccoccus aureus. International Journal of Antimicrobial Agents. 21: 279-284
Braga, L.C., Shupp, J.W., Cummings, C., Jett, M., Takahashi, J.A., Carmo, L.S., Chartone-Souza, E., Nasclmento, A.M.A. 2005. Pomegranate Extract Inhibits Staphylococcus aureus Growth and Subsequent Enterotoxin Production. Journal of Ethnopharmacology. 96 : 335-339
Nahak, M.M., Tedjasulaksana, R., Dharmawati, I.G.G.A. 2007. Khasiat Ekstrak Daun Beluntas untuk Menurunkan Jumlah Bakteri pada Saliva. Interdental Jurnal Kedokteran gigi, Denpasar. 5(3): 139-142
Nahak, M.M. 2012. Ekstrak Etanol Daun Beluntas (Pluchea indica L) dapat Mengahambat Pertumbuhan Bakteri Streptococcus mutans. Tesis. Program Pasca Sarjana Univeritas Udayana
Nahak, M.M., Tedjasulaksana, R., Sumerti, N.N. 2015. Efektivitas Obat Kumur Ekstrak Etanol Daun Beluntas (Pluchea indica L) untuk Menurunkan Jumlah Koloni Streptococcus sp. Pada Plak Gigi. Jurnal Skala Husada 12(1): 56-64
Katja, D.G., Suryanto, E., Wehantouw, F. 2009. Potensi Daun Alpukat (Persea Americana Mill) sebagai Sumber Antioksidan Alami. Chem,Prog. Vol 2(1): 58-64
Charyadie, F.L., Adi,S., Sari, R.P., 2014. Daya Hambat Ekstrak Daun Alpukat (ersea amerina Mill) Terhadap Pertumbuhan Enterococcus faecalis. Dental Jurnal Kedokteran Gigi. Vol 8 (1): 1-10
Davis, W.W., Stout,T.R. 1971. Disc Plate Methods of Microbiological Antibiotic Assay. J. Microbiology. (4):659-665.