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Comparison of generation time between Escherichia coli non- extended spectrum beta-lactamase (non-ESBL) and ESBL on ciprofloxacin and tetracycline induction: Experimental research

  • Budi Mulyawan ,
  • Agung Dwi Wahyu Widodo ,
  • Pepy Dwi Endraswari ,


Background: Escherichia coli (E. coli) is the leading cause of sepsis in the world, a country with low-middle income and the fourth largest population globally. First-generation antibiotics are still common, especially fluoroquinolones and tetracyclines, which often experience resistance in E. coli. This study aimed to analyze comparison of generation time of E. coli on ciprofloxacin and tetracycline.

Methods: This study was experimental with a posttest-only control group design. The subjects of this study were E. coli non-extended spectrum beta-lactamase (non-ESBL) and ESBL, which have ciprofloxacin of 0.25 µg/mL and tetracycline of 4 µg/mL exposed. Colony counting was performed every hour for twelve hours. The statistical test used was the independent t-test with p <0.05.

Result: E. coli non-ESBL on ciprofloxacin induction showed a generation time of 52.76 min and tetracycline of 53.66 min. However, E ESBL on ciprofloxacin induction showed a generation time of 53.96 min and tetracycline of 55.40 min. The comparison of E. coli non-ESBL exposed to ciprofloxacin and tetracycline showed no significant difference (t = 1.364; 95% CI = -0.565 – 2.352; p = 0.202) and E. coli ESBL also (t = 1.469; CI 95% = -0.748 – 3.645; p = 0.173).

Conclusion: No significant comparison exists between generation time in E. coli non-ESBL and ESBL on ciprofloxacin and tetracycline induction.


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How to Cite

Mulyawan, B. ., Widodo, A. D. W., & Endraswari, P. D. (2024). Comparison of generation time between Escherichia coli non- extended spectrum beta-lactamase (non-ESBL) and ESBL on ciprofloxacin and tetracycline induction: Experimental research. Bali Medical Journal, 13(1), 811–815.




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