Skip to main content Skip to main navigation menu Skip to site footer

Effects of Acupuncture Therapy at the Mingmen (GV4) and Zusanli (ST36) Points on the Expression of Transforming Growth Factor Beta 1 in Mice with Lupus Nephritis Model

Abstract

Introduction: Systemic Lupus Erythematosus (SLE) is a chronic autoimmune inflammation involving various factors and autoantibodies targeting cell components, resulting in epigenetic changes. Lupus nephritis is one of the clinical manifestations of SLE. Nowadays, it is known that acupuncture could inhibit disease development, including chronic nephritis, chronic diarrhea, lumbago adnexitis, impotence, enuresis in children, myelitis, and endometritis. Thus, this study aimed to examine the effect of improving Mingmen (GV4) and Zusanli (ST36) point acupuncture therapy on the expression of Transforming Growth Factor Beta 1 (TGFβ1) in lupus nephritis model mice.

Method: It was a laboratory experiment on mice (Mus musculus) lupus nephritis model with a post-test only with a control group design. The research samples were divided into four groups, with seven mice in each group, consisting of a control group (KN), a negative control group given pristane (K), treatment group 1 (P1); given pristane and methylprednisolone, and a treatment group 2 (P2); given pristane and acupuncture at the Mingmen (GV4) and Zusanli (ST36) points. This acupuncture needle insertion was carried out thrice a week for 12 insertions during the intervention. The bivariate analysis was done using Kruskal Wallis.

Results: There were 28 samples examined in this study. we found that the TGFβ1 expression was high in group P2 (72.86±11.13), after that followed by group P1 (65.71±25.73), N (60.71±25.24), and K (5.88±5.35). According to the bivariate analysis, it showed a significant difference in TGF-β (p= 0.010).

Conclusion: Mingmen (GV4) and Zusanli (ST36) point acupuncture therapy improves TGFβ1 expression. Acupuncture therapy triggers inhibition of TGF-β1 expression in lupus nephritis model mice.

References

  1. Woo JMP, Parks CG, Jacobsen S, Costenbader KH, Bernatsky S. The role of environmental exposures and gene–environment interactions in the etiology of systemic lupus erythematous. J Intern Med. 2022;291(6):755–78.
  2. Karrar S, Cunninghame Graham DS. Review: Abnormal B Cell Development in Systemic Lupus Erythematosus: What the Genetics Tell Us. Arthritis & Rheumatology. 2018;70(4):496–507.
  3. Rees F, Doherty M, Grainge MJ, Lanyon P, Zhang W. The worldwide incidence and prevalence of systemic lupus erythematosus: a systematic review of epidemiological studies. Rheumatology. 2017;56(11):1945–61.
  4. Kemenkes RI. Situasi Lupus. Jakarta; 2017.
  5. Reppe Moe SE, Molberg Ø, Strøm EH, Lerang K. Assessing the relative impact of lupus nephritis on mortality in a population-based systemic lupus erythematosus cohort. Lupus. 2019;28(7):818–25.
  6. Chang C-M, Wu P-C, Chiang J-H, Wei Y-H, Chen F-P, Chen T-J, et al. Integrative therapy decreases the risk of lupus nephritis in patients with systemic lupus erythematosus: A population-based retrospective cohort study. J Ethnopharmacol. 2017;196:201–12.
  7. Greco C, Kao A, Maksimowicz-McKinnon K, Glick R, Houze M, Sereika S, et al. Acupuncture for systemic lupus erythematosus: a pilot RCT feasibility and safety study. Lupus. 2008;17(12):1108–16.
  8. Geng X, Sun T, Li J, Zhao N, Wang Y, Yu H. Electroacupuncture in the repair of spinal cord injury: inhibiting the Notch signaling pathway and promoting neural stem cell proliferation. Neural Regen Res. 2015;10(3):394.
  9. Yang G, Zheng B, Yu Y, Huang J, Zhu H, Deng D, et al. Electroacupuncture at Zusanli (ST36), Guanyuan (CV4), and Qihai (CV6) Acupoints Regulates Immune Function in Patients with Sepsis via the PD-1 Pathway. Biomed Res Int. 2022;2022:1–9.
  10. Liu C, Zhou Z, Li B, Yang Q, Chang X, Zhou J, et al. Overview of researches on central action mechanism of needling Zusanli (ST 36). Journal of Acupuncture and Tuina Science. 2017;15(3):191–8.
  11. Xing Q, Su H, Cui J, Wang B. Role of Treg Cells and TGF-β1 in Patients with Systemic Lupus Erythematosus: A Possible Relation with Lupus Nephritis. Immunol Invest. 2012;41(1):15–27.
  12. Betül Bİ. Different Point of View to the Autoimmune Diseases and Treatment with Acupuncture. J Pharmacopuncture. 2020;23(4):187–93.
  13. Zuo Z, Huang P, Jiang Y, Zhang Y, Zhu M. Acupuncture attenuates renal interstitial fibrosis via the TGF-β/Smad pathway. Mol Med Rep. 2019;
  14. Sutanto H, Yuliasih Y. Disentangling the Pathogenesis of Systemic Lupus Erythematosus: Close Ties between Immunological, Genetic and Environmental Factors. Medicina (B Aires). 2023;59(6):1033.

How to Cite

Suparyanti, E. L., Purwanto, B., Wasita, B., & Handayani, S. (2023). Effects of Acupuncture Therapy at the Mingmen (GV4) and Zusanli (ST36) Points on the Expression of Transforming Growth Factor Beta 1 in Mice with Lupus Nephritis Model. Bali Medical Journal, 13(1), 317–321. https://doi.org/10.15562/bmj.v13i1.4871

HTML
3

Total
0

Share

Search Panel

Endang Listyaningsih Suparyanti
Google Scholar
Pubmed
BMJ Journal


Bambang Purwanto
Google Scholar
Pubmed
BMJ Journal


Brian Wasita
Google Scholar
Pubmed
BMJ Journal


Selfi Handayani
Google Scholar
Pubmed
BMJ Journal