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Increased 8-OHdG in umbilical cord blood represents the risk of preeclampsia with Intra-Uterine Growth Restriction (IUGR)

  • Andi Nurul Faizah Tenri Ola ,
  • Efendi Lukas ,
  • Deviana Soraya Riu ,
  • Rina Previana ,
  • Isharyah Sunarno ,
  • Nasrudin Andi Mappaware ,
  • Maisuri Tadjuddin Chalid ,


Abstract

Link of Video Abstract: https://youtu.be/VsAy9X8XIAk

 

Background: Preeclampsia is a pregnancy-related disease involving multi-organs, characterized by increased blood pressure in the second trimester of pregnancy. Preeclampsia can have short-term and long-term consequences for the mother and the fetus, including intra-uterine growth restriction (IUGR). One of the markers of DNA damage due to oxidative stress due to endothelial dysfunction in preeclampsia is the increase in 8-OHdG levels in cord blood. This study aims to see an increase in 8-OHdG levels in preeclampsia.

Methods: This study was a cross-sectional study with a total of 41 patients with preeclampsia and 41 patients with healthy pregnant women at Wahidin Sudirohusodo Hospital and its network. Samples were taken using the purposive sampling method according to the inclusion criteria. This 8-OHdG level was examined using the ELISA kit at the HUMRC laboratory of Hasanuddin University Hospital. The data is then processed statistically using SPSS.

Results: This study found 6 patients with PE + IUGR, 31 with PE, and 41 without PE. The examination results of 8-OHdG levels found significant differences between the 3 groups PE + IUGR, PE, and normal pregnancies, which showed a significant relationship between elevated 8-OHdG levels and the incidence of PE accompanied by IUGR (p = 0.006)

Conclusion: Elevated 8-OHdG levels are found in preeclampsia, especially in preeclampsia accompanied by IUGR.

Keywords: IUGR preeclampsia. 8-OHdG

References

  1. Mancia G, Hall JE. Introduction to a Compendium on the Pathophysiology and Treatment of Hypertension. Circ Res. 2019;124(7):967-968.
  2. Bokslag A, van Weissenbruch M, Mol BW, de Groot CJM. Preeclampsia; short and long-term consequences for mother and neonate. Early Hum Dev. 2016;102(1):47–50.
  3. Katsi V, Georgountzos G, Kallistratos MS, Zerdes I, Makris T, Manolis AJ, et al. The Role of Statins in Prevention of Preeclampsia: A Promise for the Future?. Front Pharmacol. 2017;8:247.
  4. Fox R, Kitt J, Leeson P, Aye CYL, Lewandowski AJ. Preeclampsia: Risk factors, diagnosis, management, and the cardiovascular impact on the offspring. J Clin Med. 2019;8(10):1–22.
  5. Pinheiro TV, Brunetto S, Ramos JGL, Bernardi JR, Goldani MZ. Hypertensive disorders during pregnancy and health outcomes in the offspring: a systematic review. J Dev Orig Health Dis. 2016;7(1):391–407.
  6. Warshafsky C, Pudwell J, Walker M, Wen SW, Smith GN. Prospective assessment of neurodevelopment in children following a pregnancy complicated by severe pre-eclampsia. BMJ Open. 2016;6(1):1–7.
  7. Burton GJ, Redman CW, Roberts JM, Moffett A. Pre-eclampsia : pathophysiology and clinical implications. 2019;1(1):1–15.
  8. Vakil P, Henry A, Craig ME, Gow ML. A review of infant growth and psychomotor developmental outcomes after intrauterine exposure to preeclampsia. BMC Pediatrics. 2022;22(1):1–19.
  9. Ebina S, Chiba T, Ozaki T, Kashiwakura I. Relationship between 8-hydroxydeoxyguanosine levels in placental/umbilical cord blood and maternal/neonatal obstetric factors. Experimental and Therapeutic Medicine. 2012;4(3):387–390.
  10. Kondkar AA, Azad TA, Sultan T, Osman EA, Almobarak FA, Al-Obeidan SA. Elevated Plasma Level of 8-Hydroxy-2′-deoxyguanosine Is Associated with Primary Open-Angle Glaucoma. Journal of Ophthalmology, 2020:1(1):1-10.
  11. Akinci S, Özcan HC, Balat Uǧur MG, Öztürk E, Taysi S, Sucu S. Assessment of 8-hydroxydeoxyguanosine levels in patients with preeclampsia: A prospective study. Clinical and Experimental Obstetrics and Gynecology. 2017;44(2):226–229.
  12. Fujimaki A, Watanabe K, Mori T, Kimura C, Shinohara K, Wakatsuki A. Placental oxidative DNA damage and its repair in preeclamptic women with fetal growth restriction. Placenta. 2011;32(5):367–372.
  13. Duhig K, Chappell LC, Shennan AH. Oxidative stress in pregnancy and reproduction. Obstet Med. 2016;9(1):113–116.
  14. Chiarello DI, Abad C, Rojas D, Toledo F, Vázquez CM, Mate A, et al. Oxidative stress: Normal pregnancy versus preeclampsia. Biochim Biophys Acta Mol Basis Dis. 2020;1866(2):165354.
  15. Fisher SJ. Why is placentation abnormal in preeclampsia? Am J Obstet Gynecol. 2015;213(1):S115–S122.
  16. Aouache R, Biquard L, Vaiman D, Miralles F. Oxidative Stress in Preeclampsia and Placental Diseases. Int J Mol Sci. 2018;19(5):1496.
  17. Gonzalez-Hunt CP, Wadhwa M, Sanders LH. DNA damage by oxidative stress : Measurement strategies for two genomes. Curr Opin Toxicol. 2018;7(1):87–94.
  18. Raymond D, Peterson E. A critical review of early-onset and late-onset preeclampsia. Obstetrical & gynecological survey. 2011;66(8):497-506.
  19. Chaiworapongsa T, Chaemsaithong P, Yeo L, Romero R. Pre-eclampsia part 1: current understanding of its pathophysiology. Nat Rev Nephrol. 2014;10(1):466–480.
  20. Surico D, Bordino V, Cantaluppi V, Mary D, Gentilli S, Oldani A, et al. Preeclampsia and intrauterine growth restriction: Role of human umbilical cord mesenchymal stem cells-trophoblast crosstalk. PLoS ONE. 2019;14(6):1-11.
  21. Salomon LJ, Alfirevic Z, da Silva Costa F, Deter RL, Figueras F, Ghi T, et al. ISUOG Practice Guidelines: ultrasound assessment of fetal biometry and growth. Ultrasound in Obstetrics and Gynecology. 2019;53(6):715–723.
  22. Kimura C, Watanabe K, Iwasaki A, Mori T, Matsushita H, Shinohara K et al. The severity of hypoxic changes and oxidative DNA damage in the placenta of early-onset preeclamptic women and fetal growth restriction. Journal of Maternal-Fetal and Neonatal Medicine. 2013;26(5):491–496.
  23. Bharadwaj S, Bhat VB, Vickneswaran V, Adhisivam B, Zachariah B, Habeebullah S. Oxidative stress in preeclamptic mother–newborn dyads and its correlation with early neonatal outcome–a case control study. J Matern Neonatal Med. 2018;31(1):1548–1553.
  24. Graille M, Wild P, Sauvain JJ, Hemmendinger M, Guseva Canu I, Hopf NB. Urinary 8-OHdG as a Biomarker for Oxidative Stress: A Systematic Literature Review and Meta-Analysis. Int J Mol Sci. 2020;21(11):3743.
  25. Takagi Y, Nikaido T, Toki T, Kita N, Kanai M, Ashida T, et al. Levels of oxidative stress and redox-related molecules in the placenta in preeclampsia and fetal growth restriction. Virchows Archiv. 2004;444(1):49–55.
  26. Toboła-Wróbel K, Pietryga M, Dydowicz P, Napierała M, Brązert J, Florek E. Association of Oxidative Stress on Pregnancy. Oxidative Medicine and Cellular Longevity, 2020;2020(1):1-6.
  27. Wiktor H, Kankofer M, Schmerold I, Dadak A, Lopucki M, Niedermüller, H. Oxidative DNA damage in placentas from normal and pre-eclamptic pregnancies. Virchows Archiv. 2014;445(1):74–78.
  28. Fenga C, Gangemi S, Teodoro M, Rapisarda V, Golokhvast K, Yurevich N, et al. 8-Hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to low-dose benzene. Toxicology Reports. 2017:4(5):291–295.
  29. Negi R, Pande D, Karki K, Kumar A, Khanna RS, Khanna HD. Association of oxidative DNA damage, protein oxidation and antioxidant function with oxidative stress induced cellular injury in pre-eclamptic/eclamptic mothers during fetal circulation. Chemico-Biological Interactions. 2014;208(1):77–83.
  30. Saroyo YB, Wibowo N, Irwinda R, Prijanti AR, Yunihastuti E, Bardosono S, et al. Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta. Journal of Pregnancy. 2021;1(1):1-6.
  31. Mrema D, Lie RT, Østbye T, Mahande MJ, Daltveit AK. The association between pre pregnancy body mass index and risk of preeclampsia: A registry based study from Tanzania. BMC Pregnancy and Childbirth. 2018;18(1):1-8.
  32. Bdolah Y, Elchalal U, Natanson-Yaron S, Yechiam H, Bdolah-Abram T, Greenfield CD, et al. Relationship between nulliparity and preeclampsia may be explained by altered circulating soluble fms-like tyrosine kinase 1. Hypertension in Pregnancy. 2014;33(2):250–259.
  33. Lecarpentier E, Tsatsaris V. Angiogenic balance (sFlt-1/PlGF) and preeclampsia. Ann Endocrinol (Paris). 2016;77(1):97–100.
  34. Adnyana IBP, Liwang F, Negara KS, Manuaba IBP, Bhargah A, Prabawa IPY. Clinical risk factor of preeclampsia: a five-year retrospective study in Bali Royal Hospital, Bali-Indonesia. Gineco EU. 2018;14(3):89-93.
  35. Wantania JJ, Homenta C, Kepel BJ. Relationship of Heme Oxygenase-1 (HO-1) Level with Onset and Severity in Normotensive Pregnancy and Severe Preeclampsia. Bali Medical Journal. 2016;5(1):105–109.
  36. Putra IGM, Surya IGP, Pratiwi ASIM. High Level of Soluble FMS-Like Tyrosine Kinase-1 (sFlt-1) Serum in Pregnancy as a Risk Factor of Preeclampsia. Bali Medical Journal. 2016;5(2):322–325.
  37. Masruroh NM, Rizki LK, Laili U, Alifah DN. The effectiveness of CRP and leukocyte examinations as a detection of risk factors for pre-eclampsia in pregnant women. Bali Medical Journal. 2023;12(2):1227–1230.

How to Cite

Ola, A. N. F. T., Lukas, E., Riu, D. S., Previana, R., Sunarno, I., Mappaware, N. A., & Chalid, M. T. (2023). Increased 8-OHdG in umbilical cord blood represents the risk of preeclampsia with Intra-Uterine Growth Restriction (IUGR). Bali Medical Journal, 12(3), `3026–3031. https://doi.org/10.15562/bmj.v12i3.4739
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Andi Nurul Faizah Tenri Ola
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Efendi Lukas
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Deviana Soraya Riu
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Rina Previana
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Isharyah Sunarno
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Nasrudin Andi Mappaware
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Maisuri Tadjuddin Chalid
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