One of the most common types of microbial keratitis is bacterial keratitis. The most common cause is Pseudomonas aeruginosa, which is more severe and causes local tissue damage. Fibrosis can form and cause vision loss, and perforation and spread to surrounding tissues in severe cases. Platelet-rich fibrin (PRF) membrane grafting is a new surgical technique that can optimize wound healing by preventing fibrosis formation by modifying Transforming Growth Factor Beta (TGF-β) expression and suppressing its overexpression. In addition, It preserves the structural integrity of the eye. It promotes the sustained release of growth factors, both of which are necessary for tissue regeneration and the induction of new collagen lamellae production. We suggest that in Pseudomonas aeruginosa keratitis, PRF may affect the expression of TGF- and Alpha Smooth Muscle Actin (-SMA). This literature review will reveal more details on how PRF can control inflammation and aid in Pseudomonas aeruginosa keratitis wound healing.