The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model

Nadia Hidayati; Noor Aulia Hatikhah; Winawati Eka Putri; Meidyta Sinantryana Widyaswari; David Sajid Muhammad; Nadia Nisaussholihah; Deny Febriwijaya Romadhani

doi:10.15562/bmj.v12i3.4393

The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model

Nadia Hidayati ,

Noor Aulia Hatikhah ,

Winawati Eka Putri ,

Meidyta Sinantryana Widyaswari ,

David Sajid Muhammad ,

Nadia Nisaussholihah ,

Deny Febriwijaya Romadhani ,

pdf |
DOI: https://doi.org/10.15562/bmj.v12i3.4393 |
Published: 2023-10-25

Abstract

Introduction: Indonesia is a tropical country with exposure to UV rays from the sun throughout the year, so the Indonesian population is very vulnerable to skin aging, especially extrinsic skin aging due to prolonged exposure to UV rays. This study aimed to determine the effect of long exposure to UVB rays on the thickness of the epidermis, the thickness of the dermis, and the number of blood vessels and sunburn cells in (Rattus norvegicus) Wistar strain.

Methods: This research was a post-test-only control group design using 27 rats which were divided into 3 groups, namely: control group K was untreated rats, and treatment groups P1 and P2 were rats exposed to UVB radiation for 3 weeks and 6 respectively exposure weeks. Test the effect on the study using the One-Way ANOVA test and Tukey’s Post Hoc Test.

Results: The results indicated an increase in epidermal thickness of groups P1 (44.87 μm) and P2 (56.90 μm) (significant p<0.05). A decrease in dermis thickness occurred in groups P1 (726.91 μm) and P2 (559.40 μm) (significant p<0.05). In the photoaging area, there was an increased amount of sunburn cells in group P1 (6.40) and P2 (11.40) (significant p<0.05). There was a difference in the number of blood vessels between groups K and P2 and groups P1 and P2 (p<0.05).

Conclusion: There was an effect of long exposure to UVB rays on histology in rats (Rattus norvegicus) of the photoaging model including the thickness of the epidermis layer and dermis layer, the number of blood vessels, and sunburn cells.

References

Jusuf NK, Putra IB, Muslim M. Evaluation of dermoscopic photoaging score among multiethnic in Medan, Indonesia. Bali Med J. 2022;11(3):1919–23. DOI: https://doi.org/10.15562/bmj.v11i3.3810.
Nabila Ayu Safitri, Oktavia Eka Puspita VY. Optimasi Formula Sediaan Krim Ekstrak Stroberi (Fragaria x ananassa) sebagai Krim Anti Penuaan. Maj Kesehat FKUB. 2014;1(4):235–46.
Ahmad Z, Damayanti. Penuaan Kulit : Patofisiologi dan Manifestasi Klinis. Berk Ilmu Kesehat Kulit dan Kelamin – Period Dermatology Venereol. 2018;30(03):208–15. DOI: https://doi.org/10.20473/bikk.V30.3.2018.208-215.
Choi SH, Choi S Il, Jung TD, Cho BY, Lee JH, Kim SH, et al. Anti-photoaging effect of Jeju Putgyul (unripe citrus) extracts on human dermal fibroblasts and ultraviolet B-induced hairless mouse skin. Int J Mol Sci. 2017;18(10). DOI: https://doi.org/10.3390/ijms18102052.
Adrianta KA, Sunadi Putra IMA. Utilization of Artocarpus heterophylla Lamk. As UV Protector In mice exposed to UV-B. Maj Obat Tradis. 2018;23(3):112. DOI: https://doi.org/10.22146/mot.37407.
Yano K, Kajiya K, Ishiwata M, Hong YK, Miyakawa T, Detmar M. Ultraviolet B-Induced Skin Angiogenesis Is Associated with a Switch in the Balance of Vascular Endothelial Growth Factor and Thrombospondin-1 Expression. J Invest Dermatol. 2004;122(1):201–8. DOI: https://doi.org/10.1046/j.0022-202x.2003.22101.x.
Puizina-Ivić N. Skin aging. Acta Dermatovenerologica Alpina, Pannonica Adriat. 2008;17(2):47–54.
Trojahn C, Dobos G, Lichterfeld A, Blume-Peytavi U, Kottner J. Characterizing facial skin ageing in humans: Disentangling extrinsic from intrinsic biological phenomena. Biomed Res Int. 2015;2015. DOI: https://doi.org/10.1155/2015/318586.
Peng Z, Chen B, Zheng Q, Zhu G, Cao W, Qin X, et al. Ameliorative effects of peptides from the oyster (crassostrea hongkongensis) protein hydrolysates against UVB-induced skin photodamage in mice. Mar Drugs. 2020;18(6). DOI: https://doi.org/10.3390/md18060288.
Khairuddin Djawad, Siswanto Wahab, Olivia Wibisono, Christina Avanti, Astridani R. Putranti, Agussalim Bukhari IJP. Protective Effect of Mangosteen Pericarp Extract Cream Against UVB-Induced Erythema. J Clin Aesthet Dermatol. 2020;13(10):E59–E65.
Ma W, Wlaschek M, Tantcheva-Poór I, Schneider LA, Naderi L, Razi-Wolf Z, et al. Chronological aging and photoageing of the fibroblasts and the dermal connective tissue. Clin Exp Dermatol. 2001;26(7):592–9. DOI: https://doi.org/10.1046/j.1365-2230.2001.00905.x.
Tedesco AC, Martínez L, González S. Photochemistry and photobiology of actinic erythema: Defensive and reparative cutaneous mechanisms. Brazilian J Med Biol Res. 1997;30(5):561–75. DOI: https://doi.org/10.1590/s0100-879x1997000500002.
Fan Y, Jeong JH, You GY, Park JU, Choi TH, Kim S. An Experimental Model Design for Photoaging. J Craniofac Surg. 2015;26(6):e467–71. DOI: https://doi.org/10.1097/scs.0000000000001902.
Wasliati B, W P, A W. Krim Ekstrak Bawang Hitam (Allium sativum Linn) Mencegah Peningkatan Ekspresi MMP-1 dan Penurunan Jumlah Kolagen Dermis Kulit Tikus (Rattus norvegicus) Wistar Jantan yang Dipapar Sinar UV-B. Cermin Dunia Kedokt. 2019;46(8):487–90. DOI: https://dx.doi.org/10.55175/cdk.v46i8.444.
Hachiya A, Sriwiriyanont P, Fujimura T, Ohuchi A, Kitahara T, Takema Y, et al. Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts. Am J Pathol. 2009;174(2):401–13. DOI: https://doi.org/10.2353/ajpath.2009.070500.
Yuko Ibuki, Tatsushi Toyooka, Jisshou Shirahata TO, Rensuke G. Water Soluble Fraction of Solar-Simulated Light-Exposed CrudeOil Generates Phosphorylation of Histone H2AX in Human Skin Cells Under UVA Exposure. Environ Mol Mutagen. 2007 Jul;48(6):430-9. DOI: 10.1002/em.20292. PMID: 17372987.
Levi K. UV damage and sun care: Deciphering mechanics of skin to develop next generation therapies. J Mech Behav Biomed Mater. 2013;28:471–3. DOI: http://dx.doi.org/10.1016/j.jmbbm.2013.02.008
Chung JH, Eun HC. Angiogenesis in skin aging and photoaging. J Dermatol. 2007;34(9):593–600. DOI: https://doi.org/10.1111/j.1346-8138.2007.00341.x.
Gunin AG, Petrov V V., Golubtzova NN, Vasilieva O V., Kornilova NK. Age-related changes in angiogenesis in human dermis. Exp Gerontol. 2014;55:143–51. DOI: http://dx.doi.org/10.1016/j.exger.2014.04.010
Sawane M, Kajiya K. Ultraviolet light-induced changes of lymphatic and blood vasculature in skin and their molecular mechanisms. Exp Dermatol. 2012;21(SUPPL.1):22–5. DOI: https://doi.org/10.1111/j.1600-0625.2012.01498.x.
Kajiya K, Kim YK, Kinemura Y, Kishimoto J, Chung JH. Structural alterations of the cutaneous vasculature in aged and in photoaged human skin in vivo. J Dermatol Sci. 2011;61(3):206–8. DOI: https://doi.org/10.1016/j.jdermsci.2010.12.005.

[1] Jusuf NK, Putra IB, Muslim M. Evaluation of dermoscopic photoaging score among multiethnic in Medan, Indonesia. Bali Med J. 2022;11(3):1919–23. DOI: https://doi.org/10.15562/bmj.v11i3.3810.

[2] Nabila Ayu Safitri, Oktavia Eka Puspita VY. Optimasi Formula Sediaan Krim Ekstrak Stroberi (Fragaria x ananassa) sebagai Krim Anti Penuaan. Maj Kesehat FKUB. 2014;1(4):235–46.

[3] Ahmad Z, Damayanti. Penuaan Kulit : Patofisiologi dan Manifestasi Klinis. Berk Ilmu Kesehat Kulit dan Kelamin – Period Dermatology Venereol. 2018;30(03):208–15. DOI: https://doi.org/10.20473/bikk.V30.3.2018.208-215.

[4] Choi SH, Choi S Il, Jung TD, Cho BY, Lee JH, Kim SH, et al. Anti-photoaging effect of Jeju Putgyul (unripe citrus) extracts on human dermal fibroblasts and ultraviolet B-induced hairless mouse skin. Int J Mol Sci. 2017;18(10). DOI: https://doi.org/10.3390/ijms18102052.

[5] Adrianta KA, Sunadi Putra IMA. Utilization of Artocarpus heterophylla Lamk. As UV Protector In mice exposed to UV-B. Maj Obat Tradis. 2018;23(3):112. DOI: https://doi.org/10.22146/mot.37407.

[6] Yano K, Kajiya K, Ishiwata M, Hong YK, Miyakawa T, Detmar M. Ultraviolet B-Induced Skin Angiogenesis Is Associated with a Switch in the Balance of Vascular Endothelial Growth Factor and Thrombospondin-1 Expression. J Invest Dermatol. 2004;122(1):201–8. DOI: https://doi.org/10.1046/j.0022-202x.2003.22101.x.

[7] Puizina-Ivić N. Skin aging. Acta Dermatovenerologica Alpina, Pannonica Adriat. 2008;17(2):47–54.

[8] Trojahn C, Dobos G, Lichterfeld A, Blume-Peytavi U, Kottner J. Characterizing facial skin ageing in humans: Disentangling extrinsic from intrinsic biological phenomena. Biomed Res Int. 2015;2015. DOI: https://doi.org/10.1155/2015/318586.

[9] Peng Z, Chen B, Zheng Q, Zhu G, Cao W, Qin X, et al. Ameliorative effects of peptides from the oyster (crassostrea hongkongensis) protein hydrolysates against UVB-induced skin photodamage in mice. Mar Drugs. 2020;18(6). DOI: https://doi.org/10.3390/md18060288.

[10] Khairuddin Djawad, Siswanto Wahab, Olivia Wibisono, Christina Avanti, Astridani R. Putranti, Agussalim Bukhari IJP. Protective Effect of Mangosteen Pericarp Extract Cream Against UVB-Induced Erythema. J Clin Aesthet Dermatol. 2020;13(10):E59–E65.

[11] Ma W, Wlaschek M, Tantcheva-Poór I, Schneider LA, Naderi L, Razi-Wolf Z, et al. Chronological aging and photoageing of the fibroblasts and the dermal connective tissue. Clin Exp Dermatol. 2001;26(7):592–9. DOI: https://doi.org/10.1046/j.1365-2230.2001.00905.x.

[12] Tedesco AC, Martínez L, González S. Photochemistry and photobiology of actinic erythema: Defensive and reparative cutaneous mechanisms. Brazilian J Med Biol Res. 1997;30(5):561–75. DOI: https://doi.org/10.1590/s0100-879x1997000500002.

[13] Fan Y, Jeong JH, You GY, Park JU, Choi TH, Kim S. An Experimental Model Design for Photoaging. J Craniofac Surg. 2015;26(6):e467–71. DOI: https://doi.org/10.1097/scs.0000000000001902.

[14] Wasliati B, W P, A W. Krim Ekstrak Bawang Hitam (Allium sativum Linn) Mencegah Peningkatan Ekspresi MMP-1 dan Penurunan Jumlah Kolagen Dermis Kulit Tikus (Rattus norvegicus) Wistar Jantan yang Dipapar Sinar UV-B. Cermin Dunia Kedokt. 2019;46(8):487–90. DOI: https://dx.doi.org/10.55175/cdk.v46i8.444.

[15] Hachiya A, Sriwiriyanont P, Fujimura T, Ohuchi A, Kitahara T, Takema Y, et al. Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts. Am J Pathol. 2009;174(2):401–13. DOI: https://doi.org/10.2353/ajpath.2009.070500.

[16] Yuko Ibuki, Tatsushi Toyooka, Jisshou Shirahata TO, Rensuke G. Water Soluble Fraction of Solar-Simulated Light-Exposed CrudeOil Generates Phosphorylation of Histone H2AX in Human Skin Cells Under UVA Exposure. Environ Mol Mutagen. 2007 Jul;48(6):430-9. DOI: 10.1002/em.20292. PMID: 17372987.

[17] Levi K. UV damage and sun care: Deciphering mechanics of skin to develop next generation therapies. J Mech Behav Biomed Mater. 2013;28:471–3. DOI: http://dx.doi.org/10.1016/j.jmbbm.2013.02.008

[18] Chung JH, Eun HC. Angiogenesis in skin aging and photoaging. J Dermatol. 2007;34(9):593–600. DOI: https://doi.org/10.1111/j.1346-8138.2007.00341.x.

[19] Gunin AG, Petrov V V., Golubtzova NN, Vasilieva O V., Kornilova NK. Age-related changes in angiogenesis in human dermis. Exp Gerontol. 2014;55:143–51. DOI: http://dx.doi.org/10.1016/j.exger.2014.04.010

[20] Sawane M, Kajiya K. Ultraviolet light-induced changes of lymphatic and blood vasculature in skin and their molecular mechanisms. Exp Dermatol. 2012;21(SUPPL.1):22–5. DOI: https://doi.org/10.1111/j.1600-0625.2012.01498.x.

[21] Kajiya K, Kim YK, Kinemura Y, Kishimoto J, Chung JH. Structural alterations of the cutaneous vasculature in aged and in photoaged human skin in vivo. J Dermatol Sci. 2011;61(3):206–8. DOI: https://doi.org/10.1016/j.jdermsci.2010.12.005.

The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model

Abstract

References

How to Cite

Search Panel