Skip to main content Skip to main navigation menu Skip to site footer

The effectiveness of platelet rich fibrin (PRF) graft to expression of IL-1 and TNF-α in cornea post NaOH exposure: an experimental study

  • Marisa Surya Airlangga ,
  • Reni Prastyani ,
  • Nurwasis ,
  • Hani Plumeriastuti ,
  • Budi Utomo ,


Abstract

Link of Video Abstract: https://youtu.be/Qw5E3Le-WJk

 

Background: Alkaline trauma of cornea often causes irreversible vision loss, even though it is quickly treated. The effectiveness of anti-inflammatory therapy as a main treatment is still limited clinically, as well as has dangerous side effects. Platelet-rich fibrin (PRF) is quite safe and effective in the treatment of descemetocele and also important in wound healing process. PRF membrane also provides a mechanical function as a framework for cell proliferation, differentiation and migration which is important for tissue regeneration. This study aimed to analyse the effectiveness of PRF grafts on expression of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) in cornea post NaOH exposure.

Methods: An experimental study was conducted on Oryctolagus cuniculus which was exposed to 1 N NAOH for 30 seconds. The study subjects were 14 rabbits consisting of 7 PRF graft rabbits and 7 control rabbits. The rabbit's right eye was then irrigated, dripped with 1% levofloxacin 1 drop and a PRF graft was placed.

The effectiveness of PRF graft was analysed by percentage of expression of IL-1 and TNF-α in cornea post NaOH exposure

Results: No significant differences were found in expression of IL-1 (p>0.05) and expression of TNF-α (p>0.05) in cornea post NaOH exposure. There was a decrease in the expression of IL-1 between control and PRF graft groups. The combination of the mechanical and chemotactic functions of PRF membranes makes them suitable as an autologous biomaterial for reconstruction, repair, and maintenance of ocular surfaces.

Conclusion: PRF plays a significant role in wound healing and preventing excessive fibrosis, therefore it has the enormous potential to reduce the expression of IL-1 in corneal alkaline damage.

Keywords: Platelet-rich fibrin (PRF) IL-1 TNF-α NaOH

References

  1. Nababan AR, Suryowati DI, Komaratih E, Primitasari Y, Legowo D, Notopuro PB, Notobroto HB. Platelet-rich fibrin (PRF) graft and amniotic membrane graft on transforming growth factor-β (TGF-β) and type 1 collagen post conjunctival excision. Bali Medical Journal. 2022;11(3):1744-1750.
  2. Singh P, Tyagi M, Kumar Y, Gupta KK, Sharma PD. 2013. Ocular chemical injuries and their management. Oman J. Ophthalmol. 2013;6:83.
  3. Choi SY, Kim S, Park KM. Initial healing effects of platelet-rich plasma (PRP) gel and platelet-rich fibrin (PRF) in the deep corneal wound in rabbits. Bioengineering (Basel). 2022;9(8):405.
  4. Eslani MA, Baradaran-Rafii A. Movahedan. 2014. Review article the ocular surface chemical burns. J Ophthalmol. 2014;1–7.
  5. Camacho C, Rojas E. Platelet-rich fibrin membrane for pterygium surgery: Literature review and feasibility assessment. Cureus. 2021;13(9):e17884.
  6. Mirabelli P, Peebo BB, Xeroudaki M, Koulikovska M, Lagali N. Early effects of dexamethasone and anti-VEGF therapy in an inflammatory corneal neovascularization model. Exp Eye Res. 2014;125:118–127.
  7. Şentürk F, Bahadır O, Aktaş O, Bıyık AF, Ercan E. Effects of titanium prepared platelet rich fibrin on facial nerve regeneration: an experimental study. Braz J Otorhinolaryngol. 2022;88(6):867-874.
  8. Demir A, Erdikmen DO, Altundag Y, Sevim ZT. Evaluation of surgical treatment using PRF membrane in deep corneal ulcers accompanied by a descemetocele in cats: retrospective study (2019-2021). Kafkas Universitesi Veteriner Fakultesi Dergisi. 2022;28.
  9. Ghanaati S, Booms P, Orlowska A, Kubesch A, Lorenz J, Rutkowski J, Landes C, Sader R, Kirkpatrick CJ, Choukroun J. Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. Journal of Oral Implantology. 2014;40: 679-689.
  10. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyo J, Gogly B. Platelet-rich fibron (PRF): A second-generation platelet concentrate. Part I: Technologival concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:37-44.
  11. Miron RJ, Fujioka-Kobayashi M, Bishara M, Zhang Y, Hernandez M, Choukroun J. Platelet-rich fibrin and soft tissue wound healing: a systematic review. Tissue Engineering & Regenerative Medicine International Society. 2017;23(1):83-99.
  12. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, Dohan AJ, Mouhyi J, Dohan DM. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e56-60.
  13. Bunker DJL, George RJ, Kleinschmidt A, Kumar RJ, Maitz P. Alkali-related ocular burns: a case series and review. J Burn Care Res. 2014;35:261–268.
  14. Moreno-Arrones J, Merayo-Lloves J, Varilla D. Ocular chemical burns in the workplace: epidemiological characteristics. Burns. 2020;46:1212–1218.
  15. Gustianty E, Yandi N, Rifada RM, Prahasta A. Successful rate of glaucoma surgery in uveitis glaucoma. Bali Medical Journal. 2022;11(2): 614-618.
  16. Sánchez-Ávila RM, Vázquez N, Chacón M, et al. Fibrin-plasma rich in growth factors membrane for the treatment of a rabbit alkali-burn lesion. Int J Mol Sci. 2021;22(11):5564.
  17. Wedari NLPH, Budayanti NNS, Sukrama IDM, Mayura IPB. Implementation of antinuclear antibodies in autoimmune diagnostic tests: a literature review from immunological aspects. Journal of Clinical Microbiology and Infectious Diseases. 2022;2(2):27-30.
  18. de Carvalho CKL, Fernandes BL, de Souza MA. Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: a systematic review of randomized clinical trials. J Funct Biomater. 2020;11(2):31.
  19. Arnalich F, Rodriguez AE, Luque-Rio A, Alio JL. Solid platelet rich plasma in corneal surgery. Ophthalmol Ther. 2016;5(1):31-45.
  20. Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte-and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158–167
  21. Naik, B, Karunakar P, Jayadev M, Rahul MV. 2013. Role of platelet rich fibrin in wound healing: a critical review. J Conserv Dent. 2013;16, 284–293.
  22. Vahabi S, Vaziri S, Torshabi M, Esfahrood ZR. Effects of plasma rich in growth factors and platelet-rich fibrin on proliferation and viability of human gingival fibroblasts. J Dent Tehran Univ Med Sci. 2015;12:504–512.
  23. Medeiros CS, Marino GK, Santhiago MR, Wilson SE. The corneal basement bembranes and stromal fibrosis. Investigative Ophthalmology & Visual Science. 2018;59(10), 4044–4053.
  24. Sanchez-Avila RM, Merayo-Lloves J, Riestra AC, et al. Plasma rich in growth factors membrane as coadjuvant treatment in the surgery of ocular surface disorders. Medicine (Baltimore). 2018;97(17):e0242.
  25. Guérin LP, Le-Bel G, Desjardins P, et al. The human tissue-engineered cornea (hTEC): Recent Progress. Int J Mol Sci. 2021;22(3):1291.
  26. Kamil S, Mohan RR. Corneal stromal wound healing: major regulators and therapeutic targets. Ocular Surface. 2021; 290–306.
  27. Rudner XL, Kernacki KA, Barrett RP, Hazlett LD. Prolonged elevation of IL-1 in Pseudomonas aeruginosa ocular infection regulates macrophage-inflammatory protein-2 production, polymorphonuclear neutrophil persistence, and corneal perforation. Journal of Immunology. 2000;164(12), 6576–6582.
  28. Nishida T, Saika S, Morishige N. Cornea and sclera: anatomy and physiology. Cornea. 2017;1:1–22.
  29. Sánchez-Ávila R, Vázquez N, Chacón M, Persinal-Medina M, Brea-Pastor A, Berisa-Prado S, Fernández-Vega-Cueto L, Anitua E, Meana Á, Merayo-Lloves J. Fibrin-plasma rich in growth factors membrane for the treatment of a rabbit alkali-burn lesion. Int J Mol Sci. 2021;22, 5564.
  30. Cade F, Paschalis EI, Regatieri CV, Vavvas DG, Dana R, Dohlman CH. Alkali burn to the eye: protection using TNF-α inhibition. Cornea. 2014;33, 382–389.
  31. Silva LB, dos Santos Neto AP, Maia SMAS, dos Santos Guimarães C, Quidute IL, Carvalho A, de AT, Júnior SA, Leão JC. The role of TNF-α as a proinflammatory cytokine in pathological processes. Open Dent J. 2019;13, 332–338.
  32. Ferrari G, Bignami F, Rama P. Tumor necrosis factor-α inhibitors as a treatment of corneal hemangiogenesis and lymphangiogenesis. Eye Contact Lens Sci Cli Pract. 2015;41:72–76.
  33. Hu X, Xu MX, Zhou H, Cheng S, Li F, Miao Y, Wang Z. Tumor necrosis factor-alpha aggravates gliosis and inflammation of activated retinal Müller cells. Biochem Biophys Res Commun. 2020;531, 383–389.
  34. Siska, Memed FK, Musa IR. Factors influence bullous keratopathy post cataract surgery at National Eye Center of Cicendo Eye Hospital (PMN RSMC), West Java, Indonesia. Bali Medical Journal. 2020;9(3):840-843.
  35. Alio JL, Rodriguez AE, Martinez LM, Rio AL. Autologous fibrin membrane combined with solid platelet-rich plasma in the management of perforated corneal ulcers: a pilot study. JAMA Ophthalmol. 2013; 131(6):745-51.
  36. Kim KM, Shin YT, Kim HK. Effect of autologous platelet rich plasma on persistent corneal epithelial defect after infectious keratitis. Jpn Ophthalmol. 2012; 56(6): 544-50.
  37. López-Plandolit S, Morales MC, Freire V, Etxebarría J, Durán JA. Plasma rich in growth factors as a therapeutic agent for persistent corneal epithelial defects. Cornea. 2010;29(8):843-8.
  38. Rezende MS, Silva CA, Antunes VC, Ribeiro LE, Tatsui N, Cvintal T. Uso do concentrado de plaquetas em doença da superfície ocular. Rev Bras Oftalmol. 2007; 66(4):257-61.
  39. Pezzotta S, Del Fante C, Scudeller L, Cervio M, Antoniazzi ER, Perotti C. Autologous platelet lysate for treatment of refractory ocular GVHD. Bone Marrow Transplant. 2012;47(12):1558-63.

How to Cite

Airlangga, M. S. ., Prastyani, R., Nurwasis, Plumeriastuti, H. ., & Utomo, B. . (2023). The effectiveness of platelet rich fibrin (PRF) graft to expression of IL-1 and TNF-α in cornea post NaOH exposure: an experimental study. Bali Medical Journal, 12(2), 1347–1352. https://doi.org/10.15562/bmj.v12i2.4389
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
0

Total
0

Share

Search Panel

Marisa Surya Airlangga
Google Scholar
Pubmed
BMJ Journal

Reni Prastyani
Google Scholar
Pubmed
BMJ Journal

Nurwasis
Google Scholar
Pubmed
BMJ Journal

Hani Plumeriastuti
Google Scholar
Pubmed
BMJ Journal

Budi Utomo
Google Scholar
Pubmed
BMJ Journal