Link or Video Abstract: https://youtu.be/oy1cDLwM0M8
Introduction: Periodontal packs have frequently received extra anti-inflammatory and antibiotic medications, but they might trigger allergic reactions. As a result, alternative materials are required, such as herbal plants like lime peel, which can hasten wound healing without producing negative side effects. Citrus aurantifolia Swingle's peel extract, which has active ingredients with anti-inflammatory, antioxidant, antimicrobial, and antibacterial actions, can hasten the healing of wounds when added to periodontal packs. One marker in the healing process is reducing the production of lymphocytes. This study aims to determine the effect of lime (Citrus aurantifolia Swingle) peel extract on periodontal packs and the number of lymphocytes in rabbit gingival preparations.
Methods: Thirty-two New Zealand white rabbits were employed as the sample; they were harmed on the mandibular gingiva using a punch biopsy with a diameter of 2 mm. The animals were split into eight groups on the third and fifth days, with the treatment group receiving a periodontal pack containing lime (Citrus aurantifolia Swingle) peel extract and the control group receiving a periodontal pack without it. In order to count the lymphocytes, histological preparations were examined using HE staining.
Results: Statistical test results showed a significant difference in the number of lymphocytes between the control group and the treatment group on days 3 and 5 (ANOVA, p < 0.05).
Conclusion: Lime (Citrus aurantifolia Swingle) peel extract can decrease the number of gingival lymphocytes in rabbit's periodontal tissue.