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Robusta BP-42 coffee bean extract is a new anti-tyrosinase candidate to reduce melanogenesis activity

  • Ulfa Elfiah ,
  • David Sontani Perdanakusuma ,
  • Iswinarno Doso Saputro ,
  • Misnawi ,


Background: Skin graft is one of the best options for wound closure. This method is the gold standard for skin burns and large open wounds. Coffee, or Robusta coffes (BP-2) is believed to reduce the level of hyperpigmentation due to chlorogenic acid, a major polyphenolic found in coffee with anti-tyrosinase activity. However, the anti-tyrosinase activity of BP-42 coffee extract is unknown. This study aims to measure the chlorogenic acid content of BP-42 coffee bean extract and its anti-tyrosinase activity.

Methods: BP-42 coffee beans were extracted using ethanol by maceration technique. Then, the thin-layer chromatography (TLC) profile of the BP-42 coffee bean extract was made to determine the chlorogenic acid content. At the same time, its anti-tyrosinase activity was performed using the spectrophotometric method. The chlorogenic acid's content was determined using the TLC-densitometric method. Data were analyzed using SPSS version 20.0 for Windows.

Results: BP-42 coffee bean extract had chlorogenic acid concentrations at 12.452 mg/g extract. As expected, the extract exhibited anti-tyrosinase activity with IC50 at 312.213 ppm, while kojic acid showed higher bioactivity (IC50 = 30.696 ppm).

Conclusion: BP-42 coffee bean extract showed a notable anti-tyrosinase activity, promising to be used as the natural anti-pigmentation candidate.


  1. Schlottmann F, Bucan V, Vogt PM, Krezdorn N. A short history of skin grafting in burns: From the gold standard of autologous skin grafting to the possibilities of allogeneic skin grafting with immunomodulatory approaches. Med. 2021;57(3):1–15.
  2. Janowska A, Dini V, Panduri S, Macchia M, Oranges T, Romanelli M. Epidermal skin grafting in vitiligo: a pilot study. Int Wound J. 2016;13(S3):47–51.
  3. Majid I. Grafting in vitiligo: How to get better results and how to avoid complications. J Cutan Aesthet Surg. 2013;6(2):83-89.
  4. Liodaki E, Bergmann PA, Kalousis K, Mailänder P, Siemers F. The central-pedicled intracorial skin-flap technique for reconstruction of the nipple-areola complex (NAC). Breast. 2013;22(1):74–77.
  5. Alsaif A, Karam M, Hayre A, Abul A, Aldubaikhi A, Kahlar N. Full thickness skin graft versus split thickness skin graft in paediatric patients with hand burns: Systematic review and meta-analysis. Burns. 2022;S0305-4179(22)00249-2.
  6. D'Mello SAN, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling pathways in melanogenesis. Int J Mol Sci. 2016;17(7):1–18.
  7. Chang TS. Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity. Materials (Basel). 2012;5(9):1661–1685.
  8. Qian W, Liu W, Zhu D, Cao Y, Tang A, Gong G, et al. Natural skin‑whitening compounds for the treatment of melanogenesis (Review). Exp Ther Med. 2020;20(1):173–185.
  9. Raposo G, Marks MS. Melanosomes-dark organelles enlighten endosomal membrane transport. Nat Rev Mol Cell Biol. 2007;8(10):786–797.
  10. Phasha V, Senabe J, Ndzotoyi P, Okole B, Fouche G. Chuturgoon A. Review on the Use of Kojic Acid—A Skin-Lightening Ingredient. Cosmetics. 2022;9(3):1–11.
  11. Aytemir MD, Karakay G, Ekinci D. Kojic Acid Derivatives. INTECH. 2012;(1):1-27.
  12. Index Mundi. Green Coffee Robusta Production by Country in 1000 60 KG BAGS - Country Rankings [Internet]. 2017. [Cited 5th November 2022] [Available from:]
  13. Purwanto EH, Rubiyo TJ. Karakteristik Mutu dan Citarasa Kopi Robusta Klon BP 42. BP 358 dan BP 308 Asal Bali dan Lampung. Sirinov. Balai Penelit Tanam Ind dan Penyegar. 2015;3(2):67–74.
  14. Fukushima Y, Takahashi Y, Kishimoto Y, Taguchi C, Suzuki N, Yokoyama M, et al. Consumption of polyphenols in coffee and green tea alleviates skin photoaging in healthy Japanese women. Clin Cosmet Investig Dermatol. 2020;13(2):165–72.
  15. Ribeiro HM, Allegro M, Marto J, Pedras B, Oliveira NG, Paiva A, et al. Converting Spent Coffee Grounds into Bioactive Extracts with Potential Skin Antiaging and Lightening Effects. ACS Sustain Chem Eng. 2018;6(5):6289–95.
  16. Aulifa DL, Caroline M, Tristiyanti D, Budiman A. Formulation of the serum gel containing green coffee bean (Coffea robusta l) extract as an antioxidant and tyrosinase enzyme inhibitor. Rasayan J Chem. 2020;13(4):2346–2351.
  17. Ky CL, Louarn J, Dussert S, Guyot B, Hamon S, Noirot M. Caffeine. trigonelline. chlorogenic acids and sucrose diversity in wild Coffea arabica L. and C. canephora P. accessions. Food Chem. 2001;75(2):223–230.
  18. Awwad S, Issa R, Alnsour L, Albals D, Al-Momani I. Quantification of Caffeine and Chlorogenic Acid in Green and Roasted Coffee Samples Using HPLC-DAD and Evaluation of the Effect of Degree of Roasting on Their Levels. Molecules. 2021;26(24):7502.
  19. Kath J, Byrareddy VM, Craparo A, Nguyen-Huy T, Mushtaq S, Cao L, et al. Not so robust: Robusta coffee production is highly sensitive to temperature. Glob Chang Biol. 2020;26(6):3677–3788.
  20. Verbist B, Putra AED, Budidarsono S. Factors driving land use change: Effects on watershed functions in a coffee agroforestry system in Lampung. Sumatra. Agric Syst. 2005;85(3):254–270.
  21. Wang N, Jassogne L, van Asten PJA, Mukasa D, Wanyama I, Kagezi G, et al. Evaluating coffee yield gaps and important biotic, abiotic, and management factors limiting coffee production in Uganda. Eur J Agron. 2015;63(2):1–11.
  22. Jember BK. The Amount of Rainfall (mm) By Subdistricts. Gauging Stations and Months. 2020. [Cited 16th November 2022] [Available from:]
  23. Jember P. Geografis dan Topografi – Pemerintah Kabupaten Jember. 2019. [Cited 16th November 2022] [Available from:].
  24. Yudantara IMA, Cahyani NKN, Saputra MAW, Dewi NKDP. Chlorogenic acid and kojic acid as anti-hyperpigmentation: in silico study. Pharm Reports. 2022;1(2):1-6.
  25. Kusumawati LAI, Dewi ENA, Xenograf OC, Rifrianasari K, Hidayat MA. Tyrosinase inhibition assay and skin whitening cream formulation of edamame extract (Glycine Max). Int J Pharmacogn Phytochem Res. 2015;7(6):1167–1171.
  26. Kiattisin K, Nantarat T, Leelapornpisid P. Evaluation of antioxidant and anti-tyrosinase activities as well as stability of green and roasted coffee bean extracts from Coffea arabica and Coffea canephora grown in Thailand. J Pharmacogn Phyther. 2016;8(10):182–192.
  27. Makurina ON, Zaitsev V, Kolesnikov A, Sokol O, Sadykhova A. Aging changes’ inhibition of hemostasis and blood rheological features on the background of antioxidant lipisomal preparation "Lipovitam-Beta" application. Bali Medical Journal. 2018;7(1):114–119.
  28. Carvalho Neto DPD, Gonot-Schoupinsky XP, Gonot-Schoupinsky FN. Coffee as a Naturally Beneficial and Sustainable Ingredient in Personal Care Products: A Systematic Scoping Review of the Evidence. Front Sustain. 2021;2:1-22.
  29. Xu JG, Hu QP, Liu Y. Antioxidant and DNA-Protective Activities of Chlorogenic Acid Isomers. J Agric Food Chem. 2012;60(46):11625–11630.
  30. Mulyani WRW, Sanjiwani MID, Sandra, Prabawa IPY, Lestari AAW, Wihandani DM, Suastika K, Saraswati MR, Bhargah A, Manuaba IBAP. Chaperone-Based Therapeutic Target Innovation: Heat Shock Protein 70 (HSP70) for Type 2 Diabetes Mellitus. Diabetes Metab Syndr Obes. 2020;13:559-568.
  31. Fuller M, Rao NZ. The Effect of Time. Roasting Temperature. and Grind Size on Caffeine and Chlorogenic Acid Concentrations in Cold Brew Coffee. Sci Rep. 2017;7(1):1–9.
  32. Moon JK, Hyui-Yoo SUN, Shibamoto T. Role of roasting conditions in the level of chlorogenic acid content in coffee beans: Correlation with coffee acidity. J Agric Food Chem. 2009;57(12):5365–5369.

How to Cite

Elfiah, U., David Sontani Perdanakusuma, Iswinarno Doso Saputro, & Misnawi. (2022). Robusta BP-42 coffee bean extract is a new anti-tyrosinase candidate to reduce melanogenesis activity. Bali Medical Journal, 11(3), 2022–2026.




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Ulfa Elfiah
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David Sontani Perdanakusuma
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Iswinarno Doso Saputro
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