Jatropha multifida L stem sap gel versus Aloe vera gel to post-gingivectomy healing process

Khusnul Munika Listari; Tsarwah Az-Zahra; Amalia   Hasanah; Yessy Agistasari

doi:10.15562/bmj.v12i1.3821

Jatropha multifida L stem sap gel versus Aloe vera gel to post-gingivectomy healing process

Khusnul Munika Listari ,

Tsarwah Az-Zahra ,

Amalia Hasanah ,

Yessy Agistasari ,

pdf |
DOI: https://doi.org/10.15562/bmj.v12i1.3821 |
Published: 2023-01-27

Abstract

Introduction: Gingivectomy is cutting the gingiva during a gingival enlargement condition that causes an open wound in the gingiva. Jatropha multifida L gel contains active ingredients of alkaloids, flavonoids, saponins and tannins, which are thought to accelerate the wound healing process, but its use in dentistry is limited. The objective of this research is to determine the effectiveness of the application of Jatropha multifida L stem sap gel in the wound healing process post-gingivectomy in Wistar rats (Rattus novergicus) compare with Aloe vera gel (Aloe Barbadensis Miller) by using parameters the number of fibroblasts, blood vessels and the density of collagen fibers

Methods: Rats were divided into 4 groups, namely group K (Aloe vera gel), P1 (Jatropha multifida L stem sap gel 2,5%), P2 (Jatropha multifida L stem sap gel 5%) and P3 (Jatropha multifida L stem sap gel 10%). Each group was given the gel twice a day in the wound area; then the wound was observed microscopically by Haematoxylin Eosin staining to count the number of fibroblasts and blood vessels also Masson Trichrome staining to observe the density of collagen fibers on day 3 and 7.

Results: The data obtained were statistically analyzed using the One Way Anova, Dunnet and Kruskal-Wallis test. One Way Anova test shows there is a significant difference (p<0.05) in the number of fibroblasts, and the Dunnet test shows there is a significant difference (p<0.05) in the number of fibroblasts in Jatropha multifida L stem sap gel group 2.5% and 5 % on day 3. One Way Anova test shows no significant difference (p>0.05) in the number of blood vessels. The Kruskal-Wallis test shows no significant difference (p>0.05) in collagen fiber density.

Conclusion: Jatropha multifida L Stem Sap gel has the same potential as Aloe vera gel in accelerating wound healing after gingivectomy in Wistar rats.

References

Badan Penelitian dan Pengembangan Kesehatan. Laporan Nasional Riskesdas 2018. Riset Kesehatan Dasar 2018. 2019. p. 674.
Andriani I. Perawatan Pembesaran Gingiva dengan Gingivektomi. J Mutiara Med. 2009;9(Januari):70–3.
Cohen ES. Atlas Of Cosmetic And Reconstructive Periodontal Surgery. third Edit. Hamilton, Ontario: BC Decker Inc; 2007. 39 p.
Newman M, Takei, Klokkevold, Carranza. Clinical Periodontology. Thirteenth. Carranza F, editor. Philadelphia: Elsevier; 2019. 602 p.
Sumbayak EM. Tinjauan Pustaka Fibroblas : Struktur dan Peranannya dalam Penyembuhan Luka. J Kedokt Meditek. 2015;21(6):1–6.
Yuhernita, Juniarti A. Pengaruh Pemberian Gel Dari Ekstrak Metanol Daun Jarak Tintir (Jatropha Multifida L) Terhadap Kepadatan Serabut Kolagen Dan Jumlah Angiogenesis Dalam Proses Penyembuhan Luka. In Prosiding Seminar Nasional dan Workshop “Perkembangan Terkini Sains Farmasi dan Klinik IV” tahun 2014; 2014. p. 47.
Sajjad A, Subhani Sajjad S. Aloe vera : An Ancient Herb for Modern Dentistry—A Literature Review . J Dent Surg. 2014;2014:1–6.
Thomas D, Nath MS, Mathew N, R R, Philip E, Latha MS. Alginate film modified with aloevera gel and cellulose nanocrystals for wound dressing application: Preparation, characterization and in vitro evaluation. J Drug Deliv Sci Technol. 2020;59(June):101894.
Movaffagh J, Fazli S, Taherzadeh Z. Evaluation of wound-healing efficiency of a functional Chitosan / Aloe vera hydrogel on the improvement of re-epithelialization in full thickness wound model of rat. J Tissue Viability. 2022;(July).
Babaee N, Zabihi E, Mohseni S, Moghadamnia AA. Evaluation of the therapeutic effects of Aloe vera gel on minor recurrent aphthous stomatitis. Dent Res J (Isfahan). 2012;9(4):381–5.
Hudwekar AD, Beldar A, Supriya M, Lendhey S, M T. Aloe vera on woun healing after periodontal flap surgery in chronic periodontitis patient:A randomized control trialHudwekar. J Oral Res Rev. 2019;11:72–6.
Destri C, Sudiana IK, Nugraha J. POTENSI EKSTRAK Jatropha multifida TERHADAP EKSPRESI VEGF APHTHOUS ULCER Rat norvegicus. J SainHealth. 2017;1(2):5–12.
Hirota BCK, Miyazaki CMS, Mercali CA, Verdan MC, Kalegari M, Gemin C, et al. C-glycosyl flavones and a comparative study of the antioxidant, hemolytic and toxic potential of Jatropha Multifida Leaves and bark. Int J Phytomedicine. 2012;4(1):1–5.
Sabandar CW, Ahmat N, Jaafar FM, Sahidin I. Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): A review. Phytochemistry. 2013;85:7–29.
Juniarti , Aryenti , Yuhernita , Poerwaningsih E.H , Jusuf A.A , Freisleben J S. Effects of Methanolic Jatropha Multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts. MAKARA Sci Ser. 2013;16(3):178–82.
Sahrah, Sudiana I., Nugraha J. Epitelisasi Pada Ulkus Traumatikus Oral. 2015;17(3):110–4.
Mescher AL. No Title. 14th ed. EGC, editor. Jakarta; 2017. 626 p.
Permatasari N, Handayani MP, Diah. Efek Jus Belimbing (Averrhoa carambola Linn.) dalam Meningkatkan Pembentukan Kolagen pada Soket Pasca Pencabutan Gigi Tikus Wistar. Prodenta J Dent. 2013;1:7–14.
Rizka A, Budipramana VS, Fauziah D. Kepadatan Kolagen tipe 1 pada luka operasi tikus Wistar yang mengalami anemia karena perdarahan akut. Media J Emerg. 2013;2(1):1.
Sujatha G, Senthil Kumar G, Muruganandan J, Srinivasa Prasad T. Aloe vera in dentistry. J Clin Diagnostic Res. 2014;8(10):ZI01–2.
Fatmawati CN, Retnanin E, Wahyuni TD. the Effect of Aloe vera Toward the Number of Fibroblasts on the Wound Incision of Wistar Rat'S (Ratus Norvegicus). J Vocat Nurs. 2020;1(1):30.
Kimura Y, Sumiyoshi M, Kawahira K, Sakanaka M. Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice. Br J Pharmacol. 2006;148(6):860–70.
Ivan I, Sudigdoadi S, Kartamihardja AHS. Antibacterial Effect of Jatropha Multifida L. Leaf Infusion towards Staphylococcus aureus and Pseudomonas aeruginosa. Althea Med J. 2019;6(2):95–9.
Syarfati, Eriani K, Damhoeri A. The Potential of Jarak Cina (Jatropha Multifida L.) Secretion in Healing New-Wounded Mice. J Nat. 2011;11(1):39–44.

[1] Badan Penelitian dan Pengembangan Kesehatan. Laporan Nasional Riskesdas 2018. Riset Kesehatan Dasar 2018. 2019. p. 674.

[2] Andriani I. Perawatan Pembesaran Gingiva dengan Gingivektomi. J Mutiara Med. 2009;9(Januari):70–3.

[3] Cohen ES. Atlas Of Cosmetic And Reconstructive Periodontal Surgery. third Edit. Hamilton, Ontario: BC Decker Inc; 2007. 39 p.

[4] Newman M, Takei, Klokkevold, Carranza. Clinical Periodontology. Thirteenth. Carranza F, editor. Philadelphia: Elsevier; 2019. 602 p.

[5] Sumbayak EM. Tinjauan Pustaka Fibroblas : Struktur dan Peranannya dalam Penyembuhan Luka. J Kedokt Meditek. 2015;21(6):1–6.

[6] Yuhernita, Juniarti A. Pengaruh Pemberian Gel Dari Ekstrak Metanol Daun Jarak Tintir (Jatropha Multifida L) Terhadap Kepadatan Serabut Kolagen Dan Jumlah Angiogenesis Dalam Proses Penyembuhan Luka. In Prosiding Seminar Nasional dan Workshop “Perkembangan Terkini Sains Farmasi dan Klinik IV” tahun 2014; 2014. p. 47.

[7] Sajjad A, Subhani Sajjad S. Aloe vera : An Ancient Herb for Modern Dentistry—A Literature Review . J Dent Surg. 2014;2014:1–6.

[8] Thomas D, Nath MS, Mathew N, R R, Philip E, Latha MS. Alginate film modified with aloevera gel and cellulose nanocrystals for wound dressing application: Preparation, characterization and in vitro evaluation. J Drug Deliv Sci Technol. 2020;59(June):101894.

[9] Movaffagh J, Fazli S, Taherzadeh Z. Evaluation of wound-healing efficiency of a functional Chitosan / Aloe vera hydrogel on the improvement of re-epithelialization in full thickness wound model of rat. J Tissue Viability. 2022;(July).

[10] Babaee N, Zabihi E, Mohseni S, Moghadamnia AA. Evaluation of the therapeutic effects of Aloe vera gel on minor recurrent aphthous stomatitis. Dent Res J (Isfahan). 2012;9(4):381–5.

[11] Hudwekar AD, Beldar A, Supriya M, Lendhey S, M T. Aloe vera on woun healing after periodontal flap surgery in chronic periodontitis patient:A randomized control trialHudwekar. J Oral Res Rev. 2019;11:72–6.

[12] Destri C, Sudiana IK, Nugraha J. POTENSI EKSTRAK Jatropha multifida TERHADAP EKSPRESI VEGF APHTHOUS ULCER Rat norvegicus. J SainHealth. 2017;1(2):5–12.

[13] Hirota BCK, Miyazaki CMS, Mercali CA, Verdan MC, Kalegari M, Gemin C, et al. C-glycosyl flavones and a comparative study of the antioxidant, hemolytic and toxic potential of Jatropha Multifida Leaves and bark. Int J Phytomedicine. 2012;4(1):1–5.

[14] Sabandar CW, Ahmat N, Jaafar FM, Sahidin I. Medicinal property, phytochemistry and pharmacology of several Jatropha species (Euphorbiaceae): A review. Phytochemistry. 2013;85:7–29.

[15] Juniarti , Aryenti , Yuhernita , Poerwaningsih E.H , Jusuf A.A , Freisleben J S. Effects of Methanolic Jatropha Multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts. MAKARA Sci Ser. 2013;16(3):178–82.

[16] Sahrah, Sudiana I., Nugraha J. Epitelisasi Pada Ulkus Traumatikus Oral. 2015;17(3):110–4.

[17] Mescher AL. No Title. 14th ed. EGC, editor. Jakarta; 2017. 626 p.

[18] Permatasari N, Handayani MP, Diah. Efek Jus Belimbing (Averrhoa carambola Linn.) dalam Meningkatkan Pembentukan Kolagen pada Soket Pasca Pencabutan Gigi Tikus Wistar. Prodenta J Dent. 2013;1:7–14.

[19] Rizka A, Budipramana VS, Fauziah D. Kepadatan Kolagen tipe 1 pada luka operasi tikus Wistar yang mengalami anemia karena perdarahan akut. Media J Emerg. 2013;2(1):1.

[20] Sujatha G, Senthil Kumar G, Muruganandan J, Srinivasa Prasad T. Aloe vera in dentistry. J Clin Diagnostic Res. 2014;8(10):ZI01–2.

[21] Fatmawati CN, Retnanin E, Wahyuni TD. the Effect of Aloe vera Toward the Number of Fibroblasts on the Wound Incision of Wistar Rat'S (Ratus Norvegicus). J Vocat Nurs. 2020;1(1):30.

[22] Kimura Y, Sumiyoshi M, Kawahira K, Sakanaka M. Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice. Br J Pharmacol. 2006;148(6):860–70.

[23] Ivan I, Sudigdoadi S, Kartamihardja AHS. Antibacterial Effect of Jatropha Multifida L. Leaf Infusion towards Staphylococcus aureus and Pseudomonas aeruginosa. Althea Med J. 2019;6(2):95–9.

[24] Syarfati, Eriani K, Damhoeri A. The Potential of Jarak Cina (Jatropha Multifida L.) Secretion in Healing New-Wounded Mice. J Nat. 2011;11(1):39–44.

Jatropha multifida L stem sap gel versus Aloe vera gel to post-gingivectomy healing process

Abstract

References

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