Skip to main content Skip to main navigation menu Skip to site footer

The effectivity of bovine colostrum and Mesenchymal Stem Cell (MSC) on the improvement of Alkaline Phosphatase (ALP) and Takeda G-Protein Coupled Receptor-5 (TGR5) level in post-hepatectomy Wistar rats

  • Albert Eko Hendrawijaya ,
  • Bernadus Parish Budiono ,
  • Ignatius Riwanto ,
  • Agung Putra ,
  • Erik Prabowo ,

Abstract

Background: The factor affecting the liver's healing process after resection in liver malignancy is liver fibrosis. Bovine Colostrum (BC), as one of the anti-fibrosis products of the liver, improves hepatic fibrosis and reduces hepatocyte damage caused by carbon tetrachloride (CCl4). Meanwhile, Mesenchymal Stem Cell (MSC) is a new therapeutic source after resection. MSC is able to differentiate into specific cells in the healing process. This study aims to determine the effects of BC, MSC, and a combination of both in terms of increasing Alkaline Phosphatase (ALP) and Takeda G-Protein Coupled Receptor-5 (TGR5) levels in Wistar rats post hepatectomy 50% with liver fibrosis.

Methods: This study is an experimental study with a randomized control trial design. Subjects were 25 Wistar rats (Rattus norvegicus) which were divided into 5 groups: Sham (surgery only), K (Control), K1 (CCL4 +BC), K2 (CCL4 + MSC), and K3 (CCL4 + MSC +BC). Blood was also taken to assess ALP and TGR5 at Day-3, Day-7, and Day-10 of treatment. Data were analyzed using SPSS version 25 for Windows.

Results: A significant differences are found in ALP in the Sham group with K2 (p = 0.014) and the K2 group with K1 (p=0.026) on day 10. Significant differences in TGR5 are found in the Sham and K2 groups (p=0.009), control with K2 (p=0.014), K2 with K1 (p=0.007) on day 3. On day 7 in the control group with the combination (p=0.013). The test results showed that TGR5 on day 3 had a moderate significant strong correlation on ALP levels on day 10 (r=0.596; p=0.014).

Conclusion: The combination of BC and MSC was not better than the administration of BC or MSC only in increasing ALP and TGR5 levels in rats with liver fibrosis after hepatectomy 50%.

Based on this study, the administration of MSC is recommended. 

References

  1. Martínez-Mier G, Esquivel-Torres S, Alvarado-Arenas RA, Ortiz-Bayliss AB, Lajud-Barquín FA, Zilli-Hernandez S. Liver resection morbidity, mortality, and risk factors at the departments of hepatobiliary surgery in Veracruz, Mexico.. Rev Gastroenterol Mex. 2016;81(4):195-201.
  2. van de Laarschot LF, Jansen PL, Schaap FG, Olde Damink SW. The role of bile salts in liver regeneration. Hepatol Int. 2016;10(5):733-740.
  3. Snowdon VK, Fallowfield JA. Models and mechanisms of fibrosis resolution. Alcohol Clin Exp Res. 2011;35(5):794-799.
  4. Shulman AI, Mangelsdorf DJ. Retinoid x receptor heterodimers in the metabolic syndrome. N Engl J Med. 2005;353(6):604-615.
  5. Lu TT, Makishima M, Repa JJ, Schoonjans K, Kerr TA, Auwerx J, et al. Molecular basis for feedback regulation of bile acid synthesis by nuclear receptors. Mol Cell. 2000;6(3):507-15.
  6. Redinger RN. The coming of age of our understanding of the enterohepatic circulation of bile salts. Am J Surg. 2003;185(2):168-172.
  7. Saab S, Mallam D, Cox GA 2nd, Tong MJ. Impact of coffee on liver diseases: a systematic review. Liver Int. 2014;34(4):495-504.
  8. Sun M, Kisseleva T. Reversibility of liver fibrosis. Clin Res Hepatol Gastroenterol. 2015;39 Suppl 1(0 1):S60-S63.
  9. Hagiwara K, Kataoka S, Yamanaka H, Kirisawa R, Iwai H. Detection of cytokines in bovine colostrum. Vet Immunol Immunopathol. 2000;76(3-4):183-190.
  10. Sims DE. Recent advances in pericyte biology--implications for health and disease. Can J Cardiol. 1991;7(10):431-443.
  11. Parola M, Pinzani M. Liver fibrosis: Pathophysiology, pathogenetic targets and clinical issues. Mol Aspects Med. 2019;65:37-55.
  12. Tokuyama H, Tokuyama Y, Migita S. Isolation of two new proteins from bovine colostrum which stimulate epidermal growth factor-dependent colony formation of NRK-49F cells. Growth Factors. 1990;3(2):105-114.
  13. Hurley WL, Theil PK. Perspectives on immunoglobulins in colostrum and milk. Nutrients. 2011;3(4):442-474.
  14. Afzal A, Mahmood MS, Hussain I, Akhtar M. Adulteration and microbiological quality of milk (a review). Pakistan J Nutr. 2011;10(12):1195–202.
  15. Jenny BF, Hodge SE, O'Dell GD, Ellers JE. Influence of colostrum preservation and sodium bicarbonate on performance of dairy calves. J Dairy Sci. 1984;67(2):313-318.
  16. Foley JA, Otterby DE. Availability, storage, treatment, composition, and feeding value of surplus colostrum: a review. J Dairy Sci. 1978;61(8):1033–60.
  17. Elfstrand L, Lindmark-Månsson H, Paulsson M, Nyberg L, Åkesson B. Immunoglobulins, growth factors and growth hormone in bovine colostrum and the effects of processing. Int Dairy J. 2002;12(11):879–887.
  18. Macy IG. Composition of human colostrum and milk. Am J Dis Child. 1949;78(4):589–603.
  19. Ogra SS, Weintraub D, Ogra PL. Immunologic aspects of human colostrum and milk. III. Fate and absorption of cellular and soluble components in the gastrointestinal tract of the newborn. J Immunol. 1977;119(1):245-248.
  20. Pakkanen R, Aalto J. Growth factors and antimicrobial factors of bovine colostrum. Int Dairy J. 1997;7(5):285–97.
  21. Rona ZP. Bovine colostrum emerges as immune system modulator. Am J Nat Med. 1998;3:19–23.
  22. Karthik L, Kumar G, Keswani T, Bhattacharyya A, Chandar SS, Bhaskara Rao KV. Protease inhibitors from marine actinobacteria as a potential source for antimalarial compound. PLoS One. 2014;9(3):e90972.
  23. Leclercq IA, Farrell GC, Schriemer R, Robertson GR. Leptin is essential for the hepatic fibrogenic response to chronic liver injury. J Hepatol. 2002;37(2):206-213.
  24. Driscoll J, Patel T. The mesenchymal stem cell secretome as an acellular regenerative therapy for liver disease. J Gastroenterol. 2019;54(9):763-773.
  25. Nagino M, Nimura Y, Kamiya J, Kanai M, Uesaka K, Hayakawa N, et al. Serum alkaline phosphatase after extensive liver resection: a study in patients with biliary tract carcinoma. Hepatogastroenterology. 1999;46(26):766-70.
  26. Hattori Y, Tazuma S, Yamashita G, Ochi H, Sunami Y, Nishioka T, et al. Role of phospholipase A2 in cholesterol gallstone formation is associated with biliary phospholipid species selection at the site of hepatic excretion: indirect evidence. Dig Dis Sci. 2000;45(7):1413-21.
  27. Zhang CY, Yuan WG, He P, Lei JH, Wang CX. Liver fibrosis and hepatic stellate cells: Etiology, pathological hallmarks and therapeutic targets. World J Gastroenterol. 2016;22(48):10512-10522.
  28. Sinn DH, Gwak GY, Kwon YJ, Paik SW. Anti-fibrotic effect of bovine colostrum in carbon tetrachloride- induced hepatic fibrosis. Precision and Future Medicine. 2017;1(2):88–94.
  29. Quiles JL, Ochoa JJ, Ramirez-Tortosa MC, Linde J, Bompadre S, Battino M, et al. Coenzyme Q concentration and total antioxidant capacity of human milk at different stages of lactation in mothers of preterm and full-term infants. Free Radic Res. 2006;40(2):199-206.

How to Cite

Hendrawijaya, A. E., Budiono, B. P., Riwanto, I., Putra, A., & Prabowo, E. (2021). The effectivity of bovine colostrum and Mesenchymal Stem Cell (MSC) on the improvement of Alkaline Phosphatase (ALP) and Takeda G-Protein Coupled Receptor-5 (TGR5) level in post-hepatectomy Wistar rats. Bali Medical Journal, 10(2), 824–829. https://doi.org/10.15562/bmj.v10i2.2513

HTML
1

Total
0

Share

Search Panel

Albert Eko Hendrawijaya
Google Scholar
Pubmed
BMJ Journal


Bernadus Parish Budiono
Google Scholar
Pubmed
BMJ Journal


Ignatius Riwanto
Google Scholar
Pubmed
BMJ Journal


Agung Putra
Google Scholar
Pubmed
BMJ Journal


Erik Prabowo
Google Scholar
Pubmed
BMJ Journal