ORIGINAL ARTICLE

Effectiveness of nanosilver collagen cream for healing deep dermal burns in Sprague Dawley rats: an overview of neutrophil counts and angiogenesis

Krisna Muhammad , Awal Prasetyo, Yan Wisnu Prajoko, Najatullah Najatullah, Neni Susilaningsih

Krisna Muhammad
Second Degree Student of Biomedical Science, General Surgery Resident of Medical Faculty, Universitas Diponegoro, dr. Kariadi General Hospital, Semarang, Indonesia. Email: krisquall8584@gmail.com

Awal Prasetyo
Biomedical Lecturer of Medical Faculty, Universitas Diponegoro, Semarang, Indonesia

Yan Wisnu Prajoko
Biomedical Lecturer of Medical Faculty, Universitas Diponegoro, Semarang, Indonesia

Najatullah Najatullah
Biomedical Lecturer of Medical Faculty, Universitas Diponegoro, Semarang, Indonesia

Neni Susilaningsih
Biomedical Lecturer of Medical Faculty, Universitas Diponegoro, Semarang, Indonesia
Online First: July 26, 2021 | Cite this Article
Muhammad, K., Prasetyo, A., Prajoko, Y., Najatullah, N., Susilaningsih, N. 2021. Effectiveness of nanosilver collagen cream for healing deep dermal burns in Sprague Dawley rats: an overview of neutrophil counts and angiogenesis. Bali Medical Journal 10(2): 648-652. DOI:10.15562/bmj.v10i2.2313


Background: Burn injuries remain the third cause of death in the world. The treatment, management of deep dermal burns is by applying topical medications like nanosilver collagen cream. Collagen is widely used for wound healing, cell development, angiogenesis, and platelets' activation. This study observed the effectiveness of the drug administration of nanosilver collagen cream compared to the silver sulfadiazine cream against the healing of deep dermal burns reviewed based on neutrophils counts and angiogenesis.

Methods: This study represents an experimental study of a laboratory with a randomized post-test with a control group design. The study used the Sprague Dawley mice to be divided equally into four randomly generated groups. Data were analyzed using SPSS version 20 for Windows.

Results: The Post-Hoc Mann Whitney test shows there is a significant difference in the angiogenesis between the 3rd and 6th-day groups such as group I (p = 0.034), II (P = 0.009), III (P = 0.009), and IV (P = 0.009). Kruskal-Wallis test also obtained that neutrophil counts decrease but not significant (p=0.159) on day 3rd and (p = 0.315) dan day 6th administration of nanosilver collagen.

Conclusion: The administration of nanosilver collagen creams cannot decrease neutrophils counts significantly and administration of nanosilver collagen 2% increase angiogenesis compared to the silver sulfadiazine cream against the healing of deep dermal burns reviewed from the Sprague Dawley mice.

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