The change of cell biometric and its nucleus on cervical-squamous-epithelial-cell with GA genotype of Fas-promoter-670 gene, high-risk human papillomavirus and Candida species infection: a case report

Edy Parwanto , Raditya Wratsangka, Assangga Guyansyah, Kirana Anggraeni, Reza Aditya Digambiro, David Tjahyadi, Hanslavina Arkeman, Haryo Ganeca Widyatama, Hosea Jaya Edy, Yosua Jaya Edy

Edy Parwanto
Department of Biology, Faculty of Medicine, Universitas Trisakti, Indonesia. Email:

Raditya Wratsangka
Department of Obstetry and Gynecology, Faculty of Medicine, Universitas Trisakti, Indonesia

Assangga Guyansyah
Department of Obstetry and Gynecology, Faculty of Medicine, Universitas Trisakti, Indonesia

Kirana Anggraeni
Department of Obstetry and Gynecology, Faculty of Medicine, Universitas Trisakti, Indonesia

Reza Aditya Digambiro
Department of Anatomical Pathology, Faculty of Medicine, Universitas Trisakti, Indonesia

David Tjahyadi
Department of Histology, Faculty of Medicine, Universitas Trisakti, Indonesia

Hanslavina Arkeman
Department of Histology, Faculty of Medicine, Universitas Trisakti, Indonesia

Haryo Ganeca Widyatama
Department of Histology, Faculty of Medicine, Universitas Trisakti, Indonesia

Hosea Jaya Edy
Study Program of Pharmacy, Faculty of Math and Natural Sciences, Universitas Sam Ratulangi, Indonesia

Yosua Jaya Edy
Center for Development of Civil Servants, State Personnel Board The Republic of Indonesia ; Department of Sociology, Faculty of Social Politic, Universitas Kristen Indonesia, Indonesia
Online First: April 01, 2021 | Cite this Article
Parwanto, E., Wratsangka, R., Guyansyah, A., Anggraeni, K., Digambiro, R., Tjahyadi, D., Arkeman, H., Widyatama, H., Edy, H., Edy, Y. 2021. The change of cell biometric and its nucleus on cervical-squamous-epithelial-cell with GA genotype of Fas-promoter-670 gene, high-risk human papillomavirus and Candida species infection: a case report. Bali Medical Journal 10(1): 74-81. DOI:10.15562/bmj.v10i1.2138

Background: Cervical samples of patients with human papillomavirus (HPV) infections show positive results in HPV DNA testing. HPV infection can alter cervical squamous epithelial cells (CSECs) to be abnormal. Epigenetically, CSECs that change to be abnormal are affected by Fas-promoter-670 gene polymorphism. High-risk HPV (hr-HPV) patients can be infected by other microbes, for example, Candida species (Candida sp.). The present study's purpose was to show CSEC biometrics based on epigenetics of the Fas-promoter-670 gene polymorphism in Indonesian women with hr-HPV and Candida sp. infection. Biometric quantification was performed based on the following analysis of CSECs.

Case report: Indonesian hr-HPV women at the age of 28 years, with Candida sp. infection, underwent a Pap smear examination on April 21st, 2016, using the ThinPrep method, and a blood test was also performed using the cubital vein. Blood and ThinPrep samples were examined for the Fas-promoter-670 gene polymorphism. Epigenetically, the subjects had the GA genotype of the Fas-promoter-670 gene in the blood and ThinPrep samples. Patients with Candida sp. infection in the early stages were characterized by the appearance of polymorphonuclear leukocytes (PMN), whereas those in advanced stages presented without PMN on the hyphae. CSEC biometric measurements were performed quantitatively using mononuclear CSECs (mn-CSECs) and binucleated CSECs (bn-CSECs).

Conclusion: Biometric measurements of the CSECs were performed quantitatively and assessed the length, width, area and perimeter of the cell and its nuclei. Cell length, cell width, nucleus area, nucleus perimeter and nucleus length index were significantly different between the mn-CSECs, the 1st nucleus of bn-CSECs and 2nd nucleus of bn-CSECs (P<0.05).


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