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The ethanol extract of Garcinia mangostana L peel reduces the isoniazid-induced liver damage in rats

  • Triyanta Yuli Pramana ,
  • Brian Wasita ,
  • Vitri Widyaningsih ,
  • Risya Cilmiaty ,
  • Suroto Suroto ,
  • Ambar Mudigdo ,
  • Bambang Purwanto ,


Background: The long-term use of Isoniazid (INH) as the Tuberculosis (TB) treatment increases the risk of possible liver damage. The peel from Garcinia Mangostana L. has been shown to reduce hepatotoxicity in the rat model. However, the molecular mechanism of this effect is not well-understood

Objective: This study aims to investigate the effect of the ethanol extract of the peel of Garcinia Mangostana L or mangosteen in preventing INH-induced liver damage at the cellular and molecular levels.

Methods: A total of 32 male Wistar rats (Rattus Norvegicus) three to four months old with bodyweight between 170 and 200 grams are randomly enrolled into the negative control (NC), Positive control (PC), Treatment 1, and treatment 2 groups. The rats in the positive control (PC) and the treatment groups (T1, T2) were injected with 80 mg INH per kilogram body weight to induce liver fibrosis at the beginning of the study. The rats in the negative control group (NC) are injected with normal saline. The T1 and T2 groups are given 250 mg and 500 mg per kilogram body weight of mangosteen peel ethanol extract, respectively. After 35-days of treatment, blood samples were drawn from the rats, and the SGPT level was measured. Following the sacrifice, the liver tissues were prepared into slides and immunohistochemically stained and the TGF-β1 expression and fibrosis level were examined.

Results: The expression of TGF-β1 is significantly higher in the PC and T1 groups than in the NC group (p-value <0.05). Compared with the NC groups, the SGPT level is significantly higher in the PC, T1, and T2 groups with a p-value less than 0.05. The fibrosis scoring in the T2 group is statistically lower than the PC group. (p-value <0.05)

Conclusion: The ethanol extract of Garcinia mangostana L peel at a dose of 500 mg/kg of body weight shows a significant difference in the fibrosis score between the treatment group and the positive control group.


  1. World Health Organization. Global tuberculosis report 2017. Geneva: World Health Organization; 2017.
  2. Kementerian Kesehatan Republik Indonesia. Profil kesehatan Indonesia tahun 2017. Jakarta: Kementerian Kesehatan Republik Indonesia Jalan HR; 2018.
  3. Metushi IG, Cai P, Zhu X, Nakagawa T, Uetrecht JP. A fresh look at the mechanism of isoniazid-induced hepatotoxicity. Clin Pharmacol Ther. 2011 Jun;89(6):911-4. doi:
  4. Singh D, Cho WC, Upadhyay G. Drug-Induced Liver Toxicity and Prevention by Herbal Antioxidants: An Overview. Front Physiol. 2015;6:363. doi:
  5. Naqvi IH, Mahmood K, Talib A, Mahmood A. Antituberculosis drug-induced liver injury: an ignored fact, assessment of frequency, patterns, severity and risk factors. Open J Gastroenterol. 2015;5(12):173. doi:
  6. Ahadpour M, Eskandari MR, Mashayekhi V, Haj Mohammad Ebrahim Tehrani K, Jafarian I, Naserzadeh P, et al. Mitochondrial oxidative stress and dysfunction induced by isoniazid: study on isolated rat liver and brain mitochondria. Drug Chem Toxicol. 2016;39(2):224–32. doi:
  7. Bataller R, David A. Brenner Da. Liver fibrosis J Clin Invest. 2005;115:209–18. doi:
  8. Yang L, Seki E. Toll-like receptors in liver fibrosis: cellular crosstalk and mechanisms. Front Physiol. 2012;3:138. doi:
  9. Seki E, Brenner DA. Tollâ€like receptors and adaptor molecules in liver disease: update. Hepatology. 2008;48(1):322–35. doi:
  10. Klüter T, Fitschen-Oestern S, Lippross S, Weuster M, Mentlein R, Steubesand N, et al. The antimicrobial peptide lysozyme is induced after multiple trauma. Mediators Inflamm. 2014;2014. doi:
  11. Madigan, Michael T, John M. Martinko, and Jack Parker. Brock Biology of Microorganisms. Upper Saddle River, NJ: Prentice Hall/Pearson Education; 2003.
  12. Yunitasari L. Gempur 41 Penyakit dengan Buah Manggis Khasiat dan Cara Pengolahanya untuk Pengobatan. Yogyakarta: Pustaka Baru Pres; 2011.
  13. Pedraza-Chaverri J, Cárdenas-Rodríguez N, Orozco-Ibarra M, Pérez-Rojas JM. Medicinal properties of mangosteen (Garcinia mangostana). Food Chem Toxicol. 2008;46(10):3227–39. doi:
  14. Jaisupa N, Moongkarndi P, Lomarat P, Samer J, Tunrungtavee V, Muangpaisan W, et al. Mangosteen peel extract exhibits cellular antioxidant activity by induction of catalase and heme oxygenaseâ€1 mRNA expression. J Food Biochem. 2018;42(3):e12511. doi:
  15. Widowati W, Darsono L, Suherman J, Yelliantty Y, Maesaroh M. High Performance Liquid Chromatography (HPLC) analysis, antioxidant, antiaggregation of mangosteen peel extract (Garcinia mangostana L.). Int J Biosci Biochem Bioinforma. 2014;4(6):458. doi:
  16. Fu T, Li H, Zhao Y, Cai E, Zhu H, Li P, et al. Hepatoprotective effect of α-mangostin against lipopolysaccharide/d-galactosamine-induced acute liver failure in mice. Biomed Pharmacother. 2018;106:896–901. doi:
  17. Chen L-G, Yang L-L, Wang C-C. Anti-inflammatory activity of mangostins from Garcinia mangostana. Food Chem Toxicol. 2008;46(2):688–93. doi:
  18. Wang A, Liu Q, Ye Y, Wang Y, Lin L. Identification of hepatoprotective xanthones from the pericarps of Garcinia mangostana, guided with tert-butyl hydroperoxide induced oxidative injury in HL-7702 cells. Food Funct. 2015;6(9):3013–21. doi:
  19. Goodman ZD. Grading and staging systems for inflammation and fibrosis in chronic liver diseases. J Hepatol. 2007;47(4):598–607. doi:
  20. Liu Z, Huang XR, Chen H-Y, Penninger JM, Lan HY. Loss of angiotensin-converting enzyme 2 enhances TGF-β/Smad-mediated renal fibrosis and NF-κ B-driven renal inflammation in a mouse model of obstructive nephropathy. Lab Investig. 2012;92(5):650–61. doi:
  21. Laskin DL. Gardner CR, Price VF, and Jollow DJ. Modul macrophage Funct abrogates acute Hepatotoxic acetaminophen Hepatol. 1995;21:1045–50. doi:
  22. Lan HY. Diverse roles of TGF-β/Smads in renal fibrosis and inflammation. Int J Biol Sci. 2011;7(7):1056. doi:
  23. A.J. Hanley, et al. Elevations in markers of liver injury and risk of type 2 diabetes the insulin resistance atherosclerosis study. Diabetes. 2004;53(10):2623–2632. doi:
  24. Fu T, Wang S, Liu J, Cai E, Li H, Li P, et al. Protective effects of α-mangostin against acetaminophen-induced acute liver injury in mice. Eur J Pharmacol. 2018;827:173–80. doi:
  25. Rahmaniah R, Yuyuntia Y, Soetikno V, Arozal W, Antarianto RD, Louisa M. Alpha mangostin inhibits hepatic stellate cells activation through TGF-β/smad and AKT signaling pathways: An in vitro study in LX2. Drug Res. 2018;68(3):153–8. doi:

How to Cite

Pramana, T. Y., Wasita, B., Widyaningsih, V., Cilmiaty, R., Suroto, S., Mudigdo, A., & Purwanto, B. (2021). The ethanol extract of Garcinia mangostana L peel reduces the isoniazid-induced liver damage in rats. Bali Medical Journal, 10(1), 156–159.




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