Effect of hyperbaric oxygen therapy to IFNγ and TNFα expression in pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii

Arif Rahman Nurdianto , A Aryati, Mohammad Guritno Suryokusumo, M Mufasirin

Arif Rahman Nurdianto
Doctoral Student, Universitas Airlangga; Public Health Center of Sidoarjo, Indonesia; STIKES Anwar Medika Hospital. Email: didins99@gmail.com

A Aryati
Professor in Department of Pathology Clinic, Faculty of Medicine, Universitas Airlangga, Indonesia

Mohammad Guritno Suryokusumo
Professor in Department of Hyperbaric Oxygen Therapy, Universitas Indonesia, Indonesia

M Mufasirin
Department of Parasitology, Faculty of veterinary medicine, Universitas Airlangga, Indonesia
Online First: April 01, 2019 | Cite this Article
Nurdianto, A., Aryati, A., Suryokusumo, M., Mufasirin, M. 2019. Effect of hyperbaric oxygen therapy to IFNγ and TNFα expression in pregnant Rattus novergicus infected with Tachyzoite of Toxoplasma gondii. Bali Medical Journal 8(1): 94-100. DOI:10.15562/bmj.v8i1.1316

Hyperbaric Oxygen Therapy (HBOT) is a method of increasing oxygen delivery to tissues. The therapy improves tissue oxygenation and stimulates the formation of H2O2 as a secondary messenger for Tumour Necrosis Factor alpha (TNFα), Interferon Gamma (IFNγ) and Nuclear Factor Kappa Beta phosphorylation (NF-Kb) which play an important role in the rapid transcription of a wide variety of genes in response to extracellular stimuli. This study aims to determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of IFNγ and TNFα in pregnant rats infected with Toxoplasma gondii.


To find effect of Hyperbaric Oxygen Therapy in enhancing the expressions of IFNγ and TNFα pregnant Rattus n

overgiccus infected with Toxoplasma gondii.

Patients and methods:

This study is an animal study with a ‘randomized control group of post test only design’ on 34 Rattus novergiccus Sprague Dowley rats. Randomly, the rats were divided into 4 groups. The Hyperbaric Oxygen Therapy (HBOT) treatment group A is pregnant rats infected with tachyzoite received 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the HBOT treatment group B is Pregnant only and received 10 sessions of HBOT 2.4 ATA in 3x30 minutes; the HBOT treatment group C is Pregnant and infected with tachyzoite but not received HBOT; and the group D is pregnant rats only without infection and not received HBOT. Each infected pregnant rats were given a 103 Tachyzoite of Toxoplasma gondii  via intraperitoneal injection. Examinations of IFNγ and TNFα expressions were performed on day-5 after HBOT (HBOT twice a day). Rats that die or experience abortion will be eliminated while rats that still survive will be taken the blood by intracardiac technique. IFNγ and TNFα levels were measured by serum ELISA examination.


The results shows that the Hyperbaric Oxygen Therapy can improve IFNγ (p=0.000), TNF-α (p= 0.02) significantly in the provision of HBOT 2.4 ATA for 3x30 minutes in 10 sessions over 5 days of therapy.



Based on the obtaine results,it can be conclude that HBOT can improve the expressions of IFNγ and TNFα, in the provision of HBOT 2.4 ATA for 3x30 minutes, 10 times in 5 day and HBOT administration can prevent abortion in pregnant rats infected with tachyzoite T. gondii.


Keywords: [Toxoplasma gondii, Tachyzoite, Hyperbaric Oxygen Therapy, IFNγ, TNFα]

Fundings: -



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