ORIGINAL ARTICLE

Seroepidemiological Evidence for the Presence of Japanese Encephalitis Virus Infection in Ducks, Chickens, and Pigs, Bali-Indonesia

Anak Agung Ayu Mirah Adi, Nyoman Mantik Astawa, Putu Ayu Asri Damayanti, I Made Kardena, I Gusti Made Krisna Erawan, I Wayan Suardana, I Gusti Agung Arta Putra, Yasunobu Matsumoto

Anak Agung Ayu Mirah Adi
Faculty of Veterinary Medicine Udayana University

Nyoman Mantik Astawa
Faculty of Veterinary Medicine Udayana University

Putu Ayu Asri Damayanti
Faculty of Medicine Udayana University

I Made Kardena
Faculty of Veterinary Medicine Udayana University

I Gusti Made Krisna Erawan
Faculty of Veterinary Medicine Udayana University

I Wayan Suardana
Faculty of Veterinary Medicine Udayana University

I Gusti Agung Arta Putra
Faculty of Animal Husbandry Udayana University

Yasunobu Matsumoto
Laboratory of Global Animal Resource Science, Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657-Japan.. Email: aymat@mail.ecc.u-tokyo.ac.jp
Online First: November 14, 2016 | Cite this Article
Ayu Mirah Adi, A., Astawa, N., Asri Damayanti, P., Kardena, I., Krisna Erawan, I., Suardana, I., Arta Putra, I., Matsumoto, Y. 2016. Seroepidemiological Evidence for the Presence of Japanese Encephalitis Virus Infection in Ducks, Chickens, and Pigs, Bali-Indonesia. Bali Medical Journal 5(3): 533-537. DOI:10.15562/bmj.v5i3.343


Background: The presence of various animals, such as: ducks, chickens and pigs in households increases the potential risks of zoonosis from animal to human. One of the diseases is Japanese encephalitis (JE).  The seroepidemiological studies on the presence JE among animals especially those raised in household is very important for emerging and reemerging disease control program. Ducks, chickens and pigs have long been considered as carrier and even the amplifier hosts of Japanese encephalitis virus (JEV) replication. The presence of the animal hosts and mosquitoes as vector could result in transmission of the JEV to humans. Methods: A seroepidemiological study of the presence of Japanese encephalitis virus (JEV) was conducted by collecting sera and detecting the antibody against JEV in ducks, chickens and pigs in Bali. As pig is the amplifying animal of JEV, comparison JEV antibody between ducks reared in households with pig nearby and with no pig were also determined the presence of antibody against JEV was examined by using indirect enzyme-linked immunosorbent assay (ELISA).  The serum samples with over cut off value (COV) of optical density were considered as those containing Ab against JEV. Results: Antibody against JEV was demonstrated in ducks (20.6%), chickens (36.7%) and pigs (32.2%) evaluated in this study. Moreover, there was no significant difference (p>0.05) in the prevalence of antibody against JEV in ducks kept closely with pigs compared to the antibody in the ducks reared without pigs around. Conclusion: This study convinced that antibody against JEV is found in ducks, chickens and pigs in Bali. Indicating that these animals was infected or previously infected by the virus.

No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0