ORIGINAL ARTICLE

The effect of purple sweet potato (Ipomea batatas L) ethanol extract on Estrogen Receptor Alpha (ERα) and SOD mRNA expression in the menopause-liver animal model

I Wayan Sugiritama , Ida Ayu Ika Wahyuniari, I Gusti Ayu Dewi Ratnayanti, Ni Made Linawati, I Gusti Nyoman Sri Wiryawan, Ni Luh Gede Yoni Komalasari, I Gusti Kamasan Nyoman Arijana

I Wayan Sugiritama
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia. Email: sugiritama@gmail.com

Ida Ayu Ika Wahyuniari
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

I Gusti Ayu Dewi Ratnayanti
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

Ni Made Linawati
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

I Gusti Nyoman Sri Wiryawan
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

Ni Luh Gede Yoni Komalasari
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia

I Gusti Kamasan Nyoman Arijana
Department of Histology, Faculty of Medicine, Universitas Udayana, Bali, Indonesia
Online First: October 13, 2020 | Cite this Article
Sugiritama, I., Wahyuniari, I., Ratnayanti, I., Linawati, N., Wiryawan, I., Komalasari, N., Arijana, I. 2020. The effect of purple sweet potato (Ipomea batatas L) ethanol extract on Estrogen Receptor Alpha (ERα) and SOD mRNA expression in the menopause-liver animal model. Bali Medical Journal 9(3): 538-541. DOI:10.15562/bmj.v9i3.1784


Background: Aging and estrogen deficiency in menopause women increase susceptibility to liver diseases. In postmenopausal women, the change of ERα expressions and oxidative stress is related to liver diseases' pathogenesis. Phytoestrogens have been shown to protect and repair the liver organ due to estrogen deficiency. Purple Sweet Potatoes contain anthocyanins, a subgroup of flavonoids that have been shown to have antioxidant and phytoestrogen activity. This study aims to evaluate the phytoestrogen and antioxidant activities of purple sweet potato in a liver animal model of menopause through Superoxide Dismutase (SOD) and ERα expressions.

Method: A true experimental study by randomized post-test-only control group design was conducted among 28 female Wistar rats-aged 10-12 weeks with a bodyweight of 180-220 gram, which underwent bilateral ovariectomies. These animals were divided into 4 groups, with 7 rats in each group. On day 7, Group T0, as control was given aquadest, ethanol extract of purple sweet potato was assigned to Group T1, T2, and T3 with doses 1, 2, and 4 mL/day, respectively, during 30 days. The SOD and ERα mRNA expressions were evaluated by qRT-PCR with specific primers. Data were analyzed using SPSS version 20 for Windows.

Results: The mRNA expressions of ERα were not significantly different among groups (p>0.05), while SOD expressions were significantly different among groups (p<0.05). The expression of SOD in Group T1 (504.39±231.4 pg/μL), T2 (2,147.92±417.1 pg/μL), and T3 (405.30±224.1 pg/μL) showed a significantly higher than Group T0 (15.34±15.27) (p<0.05)

Conclusion: Oxidative stress could be prevented by purple sweet potato in the liver through the SOD mRNA expression but not ERα.

References

Lee SR, Kwon SW, Lee YH, Kaya P, Kim JM, Ahn C, et al. Dietary intake of genistein suppresses hepatocellular carcinoma through AMPK-mediated apoptosis and anti-inflammation. BMC Cancer. 2019;19(1):6.

Brady CW. Liver disease in menopause. World J Gastroenterol. 2015;21(25):7613-7620.

Shimizu I, Inoue H, Yano M, Shinomiya H, Wada S, Tsuji Y, et al. Estrogen receptor levels and lipid peroxidation in hepatocellular carcinoma with hepatitis C virus infection. Liver. 2001;21(5):342-349.

Iyer JK, Kalra M, Kaul A, Payton ME, Kaul R. Estrogen receptor expression in chronic hepatitis C and hepatocellular carcinoma pathogenesis. World J Gastroenterol. 2017;23(37):6802-6816.

Cengiz M, Ozenirler S, Yılmaz G. Estrogen receptor alpha expression and liver fibrosis in chronic hepatitis C virus genotype 1b: a clinicopathological study. Hepat Mon. 2014;14(9):e21885.

Kumar P, Kale RK, Baquer NZ. Estradiol modulates membrane-linked ATPases, antioxidant enzymes, membrane fluidity, lipid peroxidation, and lipofuscin in aged rat liver. J Aging Res. 2011;2011:580245.

Zhu L, Brown WC, Cai Q, Krust A, Chambon P, McGuinness OP, et al. Estrogen treatment after ovariectomy protects against fatty liver and may improve pathway-selective insulin resistance. Diabetes. 2013;62(2):424-434.

Uebi T, Umeda M, Imai T. Estrogen induces estrogen receptor alpha expression and hepatocyte proliferation in the livers of male mice. Genes Cells. 2015;20(3):217-223.

Moreira AC, Silva AM, Santos MS, Sardão VA. Phytoestrogens as alternative hormone replacement therapy in menopause: What is real, what is unknown. J Steroid Biochem Mol Biol. 2014;143:61-71.

Thomas AJ, Ismail R, Taylor-Swanson L, et al. Effects of isoflavones and amino acid therapies for hot flashes and co-occurring symptoms during the menopausal transition and early postmenopause: a systematic review. Maturitas. 2014;78(4):263-276.

Van De Wier B, Koek GH, Bast A, Haenen GR. The potential of flavonoids in the treatment of non-alcoholic fatty liver disease. Crit Rev Food Sci Nutr. 2017;57(4):834-855.

Miquel J, Ramírez-Boscá A, Ramírez-Bosca JV, Alperi JD. Menopause: a review on the role of oxygen stress and favorable effects of dietary antioxidants. Arch Gerontol Geriatr. 2006;42(3):289-306.

Nanashima N, Horie K, Maeda H. Phytoestrogenic Activity of Blackcurrant Anthocyanins Is Partially Mediated through Estrogen Receptor Beta. Molecules. 2017;23(1):74.

Jawi I, Suprapta DN, Dwi SU, Wiwiek I. Ubi Jalar Ungu Menurunkan Kadar MDA dalam Darah dan Hati Mencit setelah Aktivitas Fisik Maksimal. Jurnal Veteriner 2008;9(2):65–72.

Wei J, Zhang G, Zhang X, Xu D, Gao J, Fan J. Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver. Plant Foods Hum Nutr. 2017;72(4):425-431.

Arjinajarn P, Chueakula N, Pongchaidecha A, Jaikumkao K, Chatsudthipong V, Mahatheeranont S, Norkaew O, et al. Anthocyanin-rich Riceberry bran extract attenuates gentamicin-induced hepatotoxicity by reducing oxidative stress, inflammation and apoptosis in rats. Biomed Pharmacother. 2017;92:412-420.

Yuwono J, Sugiritama IW, Mayun GN, Sumadi WJ. Efek pemberian ekstrak ethanol ubi jalar ungu (Ipomoea batatas L) terhadap ketebalan dan diferensiasi sel epitel vagina tikus betina yang mengalami ovariektomi. E-Jurnal Medika Udayana. 2018;7(5):203-10.

Paramita NPC, Sugiritama IW, Linawati NM, Ratnayanti IGAD, Wahyuniari IAI, Arijana IGKN, et al. Ekstrak Etanol Ubi Jalar Ungu (Ipomea Batatas L.) Menurunkan Degenerasi Lemak Jaringan Hati Tikus yang diovariektomi E-Jurnal Medika Udayana. 2019;8(1):33-9.

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611-622.

Sukocheva OA. Estrogen, estrogen receptors, and hepatocellular carcinoma: Are we there yet?. World J Gastroenterol. 2018;24(1):1-4.

Hassan MM, Botrus G, Abdel-Wahab R, Wolff RA, Li D, Tweardy D, et al. Estrogen Replacement Reduces Risk and Increases Survival Times of Women With Hepatocellular Carcinoma. Clin Gastroenterol Hepatol. 2017;15(11):1791-1799.

Mármol F, Sánchez J, López D, Martinez N, Xaus C, Peralta C, et al. Role of oxidative stress and adenosine nucleotides in the liver of aging rats. Physiol Res. 2010;59(4):553-560.

Wang X, Zhang ZF, Zheng GH, Wang AM, Sun CH, Qin SP, et al. The Inhibitory Effects of Purple Sweet Potato Color on Hepatic Inflammation Is Associated with Restoration of NAD⁺ Levels and Attenuation of NLRP3 Inflammasome Activation in High-Fat-Diet-Treated Mice. Molecules. 2017;22(8):1315.

Moldovan B, Hosu A, David L, Cimpoiu C. Total Phenolics, Total Anthocyanins, Antioxidant and Pro-oxidant Activity of Some Red Fruits Teas. Acta Chim Slov. 2016;63(2):213-219.

Muliarta M, Tirtayasa K, Prabawa PY, Wiryadana KA. Tamarillo Consumption Associated with Increased Acetylcholinesterase Activity and Improved Oxidative Stress Markers in Farmers Exposed to Daily Pesticide-related Activities in Baturiti, Bali, Indonesia. Open Access Maced J Med Sci. 2020;8(E):244-250.


No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0